In vitro activity of dicationic compounds against a North American foxhound isolate of Leishmania infantum

Alexa C. Rosypal, James E. Hall, Svetlana Bakunova, Donald A. Patrick, Stanislav Bakunov, Chad E. Stephens, Arvind Kumar, David W. Boykin, Richard R. Tidwell

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Canine leishmaniasis caused by Leishmania infantum is enzootic in the North American foxhound population. Currently available chemotherapy for canine leishmaniasis is not completely effective and relapses are common in treated dogs. Pentamidine and related aromatic diamidines possess broad spectrum antiprotozoal activity. The in vitro antileishmanial activities of 35 aromatic cationic molecules were determined, using pentamidine as the reference drug. The compounds were examined for activity against promastigotes of L. infantum isolated from a foxhound from Virginia. The compounds most active against Leishmania parasites were reversed amidines. Compound 9, a reversed amidine, exhibited the highest activity against L. infantum, with a 50% inhibitory concentration (IC50) of 0.0042 μM compared with 14.2 μM for pentamidine. Antileishmanial activities of nine compounds were at least 1000-fold higher relative to the reference drug. Results from this study indicate that several pentamidine-related compounds warrant further investigation as possible new agents for the treatment of canine leishmaniasis.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalVeterinary Parasitology
Issue number3-4
StatePublished - Apr 30 2007


  • Antileishmanial activity
  • Aromatic diamidines
  • In vitro
  • Leishmania infantum
  • Pentamidine

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)


Dive into the research topics of 'In vitro activity of dicationic compounds against a North American foxhound isolate of Leishmania infantum'. Together they form a unique fingerprint.

Cite this