TY - JOUR
T1 - In vitro toxicity study of plant latex capped silver nanoparticles in human lung carcinoma cells
AU - Valodkar, Mayur
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka C.
AU - Devkar, Ranjitsinh V.
AU - Thakore, Sonal
N1 - Funding Information:
The authors are grateful to the University Grants Commission, New Delhi for financial assistance. Special thanks to Dr. P. S. Nagar for providing Euphorbia nivulia latex for the investigation and Dr. Geeta S. Padate, Head, Department of Zoology for her encouragement.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Organically modified silver nanoparticles were prepared by biosynthetic route induced by stem latex of a medicinally important plant, Euphorbia nivulia. The reduction and stabilization is assisted by certain peptides and terpenoids present within the latex. The aqueous formulation of latex capped silver nanoparticles (LAgNPs) being completely free of toxic chemicals can be directly used for administration/in vivo delivery of nanoparticles. The in vitro cytotoxicity evaluation of the latex capped nanoparticles was carried out using human lung carcinoma cells (A549) by MTT cell viability assay. Further, possible cytotoxic mechanisms were evaluated using various biomarkers for cytotoxicity and oxidative stress viz. extracellular lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), malondialdehyde (MDA), superoxide generation and acridine orange/ethedium bromide staining. It can be concluded from the present study that LAgNP formulation is toxic to A549 cells in a dose dependent manner. Thus plant latex solubilizes the AgNPs in water and acts as a biocompatible vehicle for transport of AgNPs to tumor/cancer cells.
AB - Organically modified silver nanoparticles were prepared by biosynthetic route induced by stem latex of a medicinally important plant, Euphorbia nivulia. The reduction and stabilization is assisted by certain peptides and terpenoids present within the latex. The aqueous formulation of latex capped silver nanoparticles (LAgNPs) being completely free of toxic chemicals can be directly used for administration/in vivo delivery of nanoparticles. The in vitro cytotoxicity evaluation of the latex capped nanoparticles was carried out using human lung carcinoma cells (A549) by MTT cell viability assay. Further, possible cytotoxic mechanisms were evaluated using various biomarkers for cytotoxicity and oxidative stress viz. extracellular lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), malondialdehyde (MDA), superoxide generation and acridine orange/ethedium bromide staining. It can be concluded from the present study that LAgNP formulation is toxic to A549 cells in a dose dependent manner. Thus plant latex solubilizes the AgNPs in water and acts as a biocompatible vehicle for transport of AgNPs to tumor/cancer cells.
KW - A549 cells
KW - Biosynthesis
KW - Cytotoxicity
KW - Fluorescent microscopy
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=80054705585&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054705585&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2011.08.001
DO - 10.1016/j.msec.2011.08.001
M3 - Article
AN - SCOPUS:80054705585
SN - 0928-4931
VL - 31
SP - 1723
EP - 1728
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 8
ER -
