TY - JOUR
T1 - In vitro translation of rat lung surfactant apoprotein mRNA
AU - Katyal, Sikandar L.
AU - Singh, Gurmukh
N1 - Funding Information:
This work was supported by grants from the National Heart, Lung and Blood Institute (HL 28193) and National Cancer Institute (33717). The authors wish to acknowledge the excellent technical assistance provided by Ms. Linda Barilaro. The authors thank Ms. Linda Shab for photography and Ms. Nancy Cannon for secretarial assistance.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985/2/28
Y1 - 1985/2/28
N2 - Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.
AB - Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.
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U2 - 10.1016/S0006-291X(85)80132-7
DO - 10.1016/S0006-291X(85)80132-7
M3 - Article
C2 - 3884005
AN - SCOPUS:0021947943
SN - 0006-291X
VL - 127
SP - 106
EP - 111
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -