In vivo spin trapping of nitric oxide in mice

Andrei Komarov; David Mattson; Mark M. Jones; P. K. Singh; Ching San Lai

doi:10.1006/bbrc.1993.2170

In vivo spin trapping of nitric oxide in mice

Andrei Komarov, David Mattson, Mark M. Jones, P. K. Singh, Ching San Lai

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Abstract

We report here an in vivo spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy to measure nitric oxide (·NO) production in the blood circulation of conscious mice. The method is based on the trapping of nitric oxide (·NO) by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe²⁺) to form a stable and water-soluble [(MGD)₂-Fe²⁺-NO] complex, which gives rise to a characteristic three-line EPR spectrum of a mononitrosyl-Fe²⁺ complex (a^N= 12.5 G and g_iso=2.04) at ambient temperatures. After simultaneous intravenous injection of sodium nitroprusside (an ·NO donor) and the [(MGD)₂-Fe²⁺] complex into the lateral vein of the mouse tail, the appearance of the three-line spectrum in the blood circulation of the mouse tail was monitored continuously by using an S-band EPR spectrometer, operating at 3.5 GHz. This represents the first spin trapping of ·NO in living animals.

Original language	English (US)
Pages (from-to)	1191-1198
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	195
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.2170
State	Published - Sep 30 1993
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "In vivo spin trapping of nitric oxide in mice",

abstract = "We report here an in vivo spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy to measure nitric oxide (·NO) production in the blood circulation of conscious mice. The method is based on the trapping of nitric oxide (·NO) by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+) to form a stable and water-soluble [(MGD)2-Fe2+-NO] complex, which gives rise to a characteristic three-line EPR spectrum of a mononitrosyl-Fe2+ complex (aN= 12.5 G and giso=2.04) at ambient temperatures. After simultaneous intravenous injection of sodium nitroprusside (an ·NO donor) and the [(MGD)2-Fe2+] complex into the lateral vein of the mouse tail, the appearance of the three-line spectrum in the blood circulation of the mouse tail was monitored continuously by using an S-band EPR spectrometer, operating at 3.5 GHz. This represents the first spin trapping of ·NO in living animals.",

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AU - Komarov, Andrei

AU - Mattson, David

AU - Jones, Mark M.

AU - Singh, P. K.

AU - Lai, Ching San

PY - 1993/9/30

Y1 - 1993/9/30

N2 - We report here an in vivo spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy to measure nitric oxide (·NO) production in the blood circulation of conscious mice. The method is based on the trapping of nitric oxide (·NO) by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+) to form a stable and water-soluble [(MGD)2-Fe2+-NO] complex, which gives rise to a characteristic three-line EPR spectrum of a mononitrosyl-Fe2+ complex (aN= 12.5 G and giso=2.04) at ambient temperatures. After simultaneous intravenous injection of sodium nitroprusside (an ·NO donor) and the [(MGD)2-Fe2+] complex into the lateral vein of the mouse tail, the appearance of the three-line spectrum in the blood circulation of the mouse tail was monitored continuously by using an S-band EPR spectrometer, operating at 3.5 GHz. This represents the first spin trapping of ·NO in living animals.

AB - We report here an in vivo spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy to measure nitric oxide (·NO) production in the blood circulation of conscious mice. The method is based on the trapping of nitric oxide (·NO) by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+) to form a stable and water-soluble [(MGD)2-Fe2+-NO] complex, which gives rise to a characteristic three-line EPR spectrum of a mononitrosyl-Fe2+ complex (aN= 12.5 G and giso=2.04) at ambient temperatures. After simultaneous intravenous injection of sodium nitroprusside (an ·NO donor) and the [(MGD)2-Fe2+] complex into the lateral vein of the mouse tail, the appearance of the three-line spectrum in the blood circulation of the mouse tail was monitored continuously by using an S-band EPR spectrometer, operating at 3.5 GHz. This represents the first spin trapping of ·NO in living animals.

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In vivo spin trapping of nitric oxide in mice

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