Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

Takao Yasuhara; Koichi Hara; Kapil D. Sethi; John C. Morgan; Cesario V. Borlongan

doi:10.1016/j.brainres.2006.11.072

Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

Takao Yasuhara, Koichi Hara, Kapil D. Sethi, John C. Morgan, Cesario V. Borlongan

Neurology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

Original language	English (US)
Pages (from-to)	49-52
Number of pages	4
Journal	Brain Research
Volume	1133
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2006.11.072
State	Published - Jan 16 2007

Keywords

8-OHdG
Biomarker
Neurodegeneration
Oxidative stress
Parkinson's disease

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2006.11.072

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title = "Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats",

abstract = "8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.",

keywords = "8-OHdG, Biomarker, Neurodegeneration, Oxidative stress, Parkinson's disease",

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AU - Hara, Koichi

AU - Sethi, Kapil D.

AU - Morgan, John C.

AU - Borlongan, Cesario V.

PY - 2007/1/16

Y1 - 2007/1/16

N2 - 8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

AB - 8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

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Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

Abstract

Keywords

ASJC Scopus subject areas

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