Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation

Deeksha Saxena; Sharad B. Purohit; G. Pradeep Kumar; Malini Laloraya

doi:10.1006/niox.2000.0286

Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation

Deeksha Saxena, Sharad B. Purohit, G. Pradeep Kumar, Malini Laloraya

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

The aim of this study was to investigate the presence of iNOS in the murine uterus and embryo at implantation. Western blot analysis showed the presence of a 130-kDa band with strong reactivity to anti-iNOS antibody in the pre- and peri-implantation stage uteri. This band was faint in the postimplantation uteri. Immunocytochemical studies showed a heavy localization of iNOS specifically on the apical cells of the uterine endometrium in the pre- and peri-implantation stages. But the postimplantation uteri showed resorbed endometrium showing weaker expression of iNOS. The iNOS was induced by estrogen and the induction was intensified when progesterone was given along with estrogen. This truly mimics the in vivo situation since implantation in mice occurs when an estrogen surge occurs on a background of progesterone. The embryos too express iNOS at the peri-implantation stage. We suggest that iNOS expressed at peri-implantation would lead to enhanced NO production, which could act as a vasodilator and an angiogenic mediator. These effects could promote the attachment of the blastocyst to the uterus. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	384-391
Number of pages	8
Journal	Nitric Oxide - Biology and Chemistry
Volume	4
Issue number	4
DOIs	https://doi.org/10.1006/niox.2000.0286
State	Published - 2000
Externally published	Yes

Keywords

Blastocyst
Embryo implantation
NO
O/·
iNOS

ASJC Scopus subject areas

Biochemistry
Physiology
Clinical Biochemistry
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/niox.2000.0286

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title = "Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation",

abstract = "The aim of this study was to investigate the presence of iNOS in the murine uterus and embryo at implantation. Western blot analysis showed the presence of a 130-kDa band with strong reactivity to anti-iNOS antibody in the pre- and peri-implantation stage uteri. This band was faint in the postimplantation uteri. Immunocytochemical studies showed a heavy localization of iNOS specifically on the apical cells of the uterine endometrium in the pre- and peri-implantation stages. But the postimplantation uteri showed resorbed endometrium showing weaker expression of iNOS. The iNOS was induced by estrogen and the induction was intensified when progesterone was given along with estrogen. This truly mimics the in vivo situation since implantation in mice occurs when an estrogen surge occurs on a background of progesterone. The embryos too express iNOS at the peri-implantation stage. We suggest that iNOS expressed at peri-implantation would lead to enhanced NO production, which could act as a vasodilator and an angiogenic mediator. These effects could promote the attachment of the blastocyst to the uterus. (C) 2000 Academic Press.",

keywords = "Blastocyst, Embryo implantation, NO, O/·, iNOS",

author = "Deeksha Saxena and Purohit, {Sharad B.} and {Pradeep Kumar}, G. and Malini Laloraya",

note = "Funding Information: This work was supported by CSIR Grant No. 37(0871)/95-EMR-II to P.K.G. and CSIR Sanction No. 13(7221-A)/97-Pool to M.L. D.S. is the recipient of a senior research fellowship from ICMR Project No. 3/1/2/6/99-RHN. S.B.P. was the recipient of a senior research fellowship from CSIR.",

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language = "English (US)",

volume = "4",

pages = "384--391",

journal = "Nitric Oxide - Biology and Chemistry",

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AU - Saxena, Deeksha

AU - Purohit, Sharad B.

AU - Pradeep Kumar, G.

AU - Laloraya, Malini

N1 - Funding Information: This work was supported by CSIR Grant No. 37(0871)/95-EMR-II to P.K.G. and CSIR Sanction No. 13(7221-A)/97-Pool to M.L. D.S. is the recipient of a senior research fellowship from ICMR Project No. 3/1/2/6/99-RHN. S.B.P. was the recipient of a senior research fellowship from CSIR.

PY - 2000

Y1 - 2000

N2 - The aim of this study was to investigate the presence of iNOS in the murine uterus and embryo at implantation. Western blot analysis showed the presence of a 130-kDa band with strong reactivity to anti-iNOS antibody in the pre- and peri-implantation stage uteri. This band was faint in the postimplantation uteri. Immunocytochemical studies showed a heavy localization of iNOS specifically on the apical cells of the uterine endometrium in the pre- and peri-implantation stages. But the postimplantation uteri showed resorbed endometrium showing weaker expression of iNOS. The iNOS was induced by estrogen and the induction was intensified when progesterone was given along with estrogen. This truly mimics the in vivo situation since implantation in mice occurs when an estrogen surge occurs on a background of progesterone. The embryos too express iNOS at the peri-implantation stage. We suggest that iNOS expressed at peri-implantation would lead to enhanced NO production, which could act as a vasodilator and an angiogenic mediator. These effects could promote the attachment of the blastocyst to the uterus. (C) 2000 Academic Press.

AB - The aim of this study was to investigate the presence of iNOS in the murine uterus and embryo at implantation. Western blot analysis showed the presence of a 130-kDa band with strong reactivity to anti-iNOS antibody in the pre- and peri-implantation stage uteri. This band was faint in the postimplantation uteri. Immunocytochemical studies showed a heavy localization of iNOS specifically on the apical cells of the uterine endometrium in the pre- and peri-implantation stages. But the postimplantation uteri showed resorbed endometrium showing weaker expression of iNOS. The iNOS was induced by estrogen and the induction was intensified when progesterone was given along with estrogen. This truly mimics the in vivo situation since implantation in mice occurs when an estrogen surge occurs on a background of progesterone. The embryos too express iNOS at the peri-implantation stage. We suggest that iNOS expressed at peri-implantation would lead to enhanced NO production, which could act as a vasodilator and an angiogenic mediator. These effects could promote the attachment of the blastocyst to the uterus. (C) 2000 Academic Press.

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Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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