Induced DNA recombination by Cre recombinase protein transduction

Sunil K. Joshi; Kahoko Hashimoto; Pandelakis A. Koni

doi:10.1002/gene.10089

Induced DNA recombination by Cre recombinase protein transduction

Sunil K. Joshi, Kahoko Hashimoto, Pandelakis A. Koni

Infectious Disease

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.

Original language	English (US)
Pages (from-to)	48-54
Number of pages	7
Journal	Genesis
Volume	33
Issue number	1
DOIs	https://doi.org/10.1002/gene.10089
State	Published - 2002

Keywords

Cre recombinase
Gene deletion
Induced DNA recombination
Protein transduction
TAT

ASJC Scopus subject areas

Genetics
Endocrinology
Cell Biology

Access to Document

10.1002/gene.10089

Cite this

@article{e1b28586a05d4c579cd945b8a845434a,

title = "Induced DNA recombination by Cre recombinase protein transduction",

abstract = "Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.",

keywords = "Cre recombinase, Gene deletion, Induced DNA recombination, Protein transduction, TAT",

author = "Joshi, {Sunil K.} and Kahoko Hashimoto and Koni, {Pandelakis A.}",

year = "2002",

doi = "10.1002/gene.10089",

language = "English (US)",

volume = "33",

pages = "48--54",

journal = "Genesis",

issn = "1526-954X",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - Induced DNA recombination by Cre recombinase protein transduction

AU - Joshi, Sunil K.

AU - Hashimoto, Kahoko

AU - Koni, Pandelakis A.

PY - 2002

Y1 - 2002

N2 - Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.

AB - Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.

KW - Cre recombinase

KW - Gene deletion

KW - Induced DNA recombination

KW - Protein transduction

KW - TAT

UR - http://www.scopus.com/inward/record.url?scp=0036274381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036274381&partnerID=8YFLogxK

U2 - 10.1002/gene.10089

DO - 10.1002/gene.10089

M3 - Article

C2 - 12001069

AN - SCOPUS:0036274381

SN - 1526-954X

VL - 33

SP - 48

EP - 54

JO - Genesis

JF - Genesis

IS - 1

ER -

Induced DNA recombination by Cre recombinase protein transduction

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this