Abstract
Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 48-54 |
| Number of pages | 7 |
| Journal | Genesis (United States) |
| Volume | 33 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2002 |
Keywords
- Cre recombinase
- Gene deletion
- Induced DNA recombination
- Protein transduction
- TAT
ASJC Scopus subject areas
- Genetics
- Endocrinology
- Cell Biology
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