TY - JOUR
T1 - Induction of murine hepatic glutathione S-transferase by dietary dehydroepiandrosterone
AU - Milewich, Leon
AU - Marrero, Mario
AU - Tezabwala, Bilquis U.
AU - Bennett, Michael
AU - Frenkel, Rene A.
AU - Slaughter, Clive A.
N1 - Funding Information:
Acknowledgements--The authors wish to thank Carolyn R. Moomaw and Joan Hsu for expert technical assistance. This investigation was supported, in part, by USPHS Grant U01 CA43311.
PY - 1993/9
Y1 - 1993/9
N2 - The naturally occurring steroid dehydroepiandrosterone (DHEA), when administered as a supplement to the diet of mice and rats, produces alterations in the relative concentrations of specific liver proteins; among these, a protein of Mr ∼ 28 K is markedly induced by DHEA action. In the present study we identified the murine hepatic ∼ 28 kDa protein as glutathione S-transferase subtype GT-8.7. Glutathione S-transferases belong to a gene superfamily that encode closely related proteins which are induced in liver and other tissues by various chemicals, including carcinogens and chemoprotective agents such as dietary antioxidants. Based on the above finding, we evaluated glutathione S-transferase activity in cytosols and microsomes prepared from liver tissue of mice fed either a control diet or a DHEA-containing diet (0.45%, by weight). The specific activity of hepatic cytosolic glutathione S-transferase in mice treated with DHEA up to 7 days was either unchanged or slightly decreased when compared to controls; however, treatment for 14 days or longer resulted in significant increases in activity. The specific activity of microsomal glutathione S-transferase also was increased by long-term DHEA treatment; however, its activity was approximately one-tenth of that in corresponding cytosols.
AB - The naturally occurring steroid dehydroepiandrosterone (DHEA), when administered as a supplement to the diet of mice and rats, produces alterations in the relative concentrations of specific liver proteins; among these, a protein of Mr ∼ 28 K is markedly induced by DHEA action. In the present study we identified the murine hepatic ∼ 28 kDa protein as glutathione S-transferase subtype GT-8.7. Glutathione S-transferases belong to a gene superfamily that encode closely related proteins which are induced in liver and other tissues by various chemicals, including carcinogens and chemoprotective agents such as dietary antioxidants. Based on the above finding, we evaluated glutathione S-transferase activity in cytosols and microsomes prepared from liver tissue of mice fed either a control diet or a DHEA-containing diet (0.45%, by weight). The specific activity of hepatic cytosolic glutathione S-transferase in mice treated with DHEA up to 7 days was either unchanged or slightly decreased when compared to controls; however, treatment for 14 days or longer resulted in significant increases in activity. The specific activity of microsomal glutathione S-transferase also was increased by long-term DHEA treatment; however, its activity was approximately one-tenth of that in corresponding cytosols.
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U2 - 10.1016/0960-0760(93)90221-H
DO - 10.1016/0960-0760(93)90221-H
M3 - Article
C2 - 9831480
AN - SCOPUS:0027482263
SN - 0960-0760
VL - 46
SP - 321
EP - 329
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 3
ER -