Infection of Aedes aegypti mosquitoes with midgut-attenuated sindbis virus reduces, but does not eliminate, disseminated infection

Alexis Carpenter, William Bart Bryant, Scott R. Santos, Rollie J. Clem

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Arboviruses are transmitted by specific vectors, and the reasons for this specificity are not fully understood. One contributing factor is the existence of tissue barriers within the vector such as the midgut escape barrier. We used microRNA (miRNA) targeting of Sindbis virus (SINV) to study how replication in midgut cells contributes to overcoming this barrier in the mosquito Aedes aegypti. SINV constructs were designed to be attenuated specifically in midgut cells by inserting binding sites for midgut-specific miRNAs into either the 39 untranslated region (MRE39miRT) or the structural open reading frame (MRE-ORFmiRT) of the SINV genome. Both miRNA-targeted viruses replicated less efficiently than control viruses in the presence of these miRNAs. When mosquitoes were given infectious blood meals containing miRNA-targeted viruses, only around 20% (MRE39miRT) or 40% (MREORFmiRT) of mosquitoes developed disseminated infection. In contrast, dissemination occurred in almost all mosquitoes fed control viruses. Deep sequencing of virus populations from individual mosquitoes ruled out selection for mutations in the inserted target sequences as the cause for dissemination in these mosquitoes. In mosquitoes that became infected with miRNA-targeted viruses, titers were equivalent to those of mosquitoes infected with control virus in both the midgut and the carcass, and there was no evidence of a threshold titer necessary for dissemination. Instead, it appeared that if infection was successfully established in the midgut, replication and dissemination were largely normal. Our results support the hypothesis that replication is an important factor in allowing SINV to overcome the midgut escape barrier but hint that other factors are also likely involved.

Original languageEnglish (US)
Article numbere00136-21
JournalJournal of Virology
Issue number13
StatePublished - Jun 2021
Externally publishedYes


  • Arbovirus
  • MiRNA targeting
  • Midgut escape barrier
  • Tissue-specific attenuation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


Dive into the research topics of 'Infection of Aedes aegypti mosquitoes with midgut-attenuated sindbis virus reduces, but does not eliminate, disseminated infection'. Together they form a unique fingerprint.

Cite this