Influence of elevated renin substrate on angiotensin II and arterial blood pressure in conscious mice

Brian C. Cholewa, David L. Mattson

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The present experiments were performed to determine the influence of intravenous administration of renin substrate on plasma angiotensin II levels and mean arterial blood pressure in conscious C57BL/6J mice. Mice with chronic indwelling femoral arterial and venous catheters were acutely or chronically administered intravenous doses of a synthetic peptide corresponding to the 14 amino acids on the N-terminal of angiotensinogen. A dose-dependent increase in arterial blood pressure was observed as the intravenous bolus dose of the renin substrate was increased from 0.18 to 180 nmol kg-1 with a maximal increase in pressure of 40 ± 3 mmHg achieved following administration of the 18 nmol kg-1 bolus (n = 11). Additional experiments demonstrated that a sustained intravenous infusion of the renin substrate led to a long-term increase in arterial blood pressure. The continuous infusion of renin substrate at 0.05 nmol kg-1 min-1 for 3 days did not alter arterial blood pressure from the control level of 119 ± 5 mmHg (n = 5); however, arterial blood pressure significantly increased to 129 ± 6 mmHg with an infusion rate of 0.5 nmol kg-1 min-1 and further increased to 141 ± 3 mmHg when the renin substrate infusion was increased to 5.0 nmol kg-1 min-1. Finally, the infusion of renin substrate at 5.0 nmol kg-1 min-1 resulted in a significant increase in plasma angiotensin II concentration from 34 ± 6 pg ml-1 in vehicle-infused mice to 288 ± 109 pg ml-1. These results demonstrate that modulation of the circulating level of angiotensinogen can alter the plasma angiotensin II level and arterial blood pressure in normal animals.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalExperimental Physiology
Issue number4
StatePublished - Jul 2005
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)


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