Abstract
Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-71 |
| Number of pages | 7 |
| Journal | Chinese Journal of Medical Genetics |
| Volume | 17 |
| Issue number | 2 |
| State | Published - 2000 |
| Externally published | Yes |
Keywords
- Heterozygosity
- Microsatellite locus
- Polymerase chain reaction
- Polymorphism information content
ASJC Scopus subject areas
- Genetics(clinical)