Information behavior of microsatellite loci in genome scanning

W. Yuan; Hongyan Xu; J. Zhao; W. Ding; H. Jiang; M. Gu; J. Xue; J. Chen; F. Fang; Z. Chen; L. Jin; W. Huang

Information behavior of microsatellite loci in genome scanning

W. Yuan
, Hongyan Xu
, J. Zhao
, W. Ding
, H. Jiang
, M. Gu
, J. Xue
, J. Chen
, F. Fang
, Z. Chen
, L. Jin
, W. Huang

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.

Original language	English (US)
Pages (from-to)	65-71
Number of pages	7
Journal	Chinese Journal of Medical Genetics
Volume	17
Issue number	2
State	Published - 2000
Externally published	Yes

Keywords

Heterozygosity
Microsatellite locus
Polymerase chain reaction
Polymorphism information content

ASJC Scopus subject areas

Genetics(clinical)

Cite this

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title = "Information behavior of microsatellite loci in genome scanning",

abstract = "Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88\% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95\% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.",

keywords = "Heterozygosity, Microsatellite locus, Polymerase chain reaction, Polymorphism information content",

author = "W. Yuan and Hongyan Xu and J. Zhao and W. Ding and H. Jiang and M. Gu and J. Xue and J. Chen and F. Fang and Z. Chen and L. Jin and W. Huang",

year = "2000",

language = "English (US)",

volume = "17",

pages = "65--71",

journal = "Chinese Journal of Medical Genetics",

issn = "1003-9406",

publisher = "Chinese Medical Journals Publishing House Co. Ltd",

number = "2",

}

TY - JOUR

T1 - Information behavior of microsatellite loci in genome scanning

AU - Yuan, W.

AU - Xu, Hongyan

AU - Zhao, J.

AU - Ding, W.

AU - Jiang, H.

AU - Gu, M.

AU - Xue, J.

AU - Chen, J.

AU - Fang, F.

AU - Chen, Z.

AU - Jin, L.

AU - Huang, W.

PY - 2000

Y1 - 2000

N2 - Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.

AB - Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.

KW - Heterozygosity

KW - Microsatellite locus

KW - Polymerase chain reaction

KW - Polymorphism information content

UR - https://www.scopus.com/pages/publications/0034092793

UR - https://www.scopus.com/pages/publications/0034092793#tab=citedBy

M3 - Article

C2 - 10751522

AN - SCOPUS:0034092793

SN - 1003-9406

VL - 17

SP - 65

EP - 71

JO - Chinese Journal of Medical Genetics

JF - Chinese Journal of Medical Genetics

IS - 2

ER -

Information behavior of microsatellite loci in genome scanning

Abstract

Keywords

ASJC Scopus subject areas

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