Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells

Mario B. Marrero; Virginia J. Venema; Han He; Ruth B. Caldwell; Richard C. Venema

doi:10.1006/bbrc.1998.9678

Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells

Mario B. Marrero, Virginia J. Venema, Han He, Ruth B. Caldwell, Richard C. Venema

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Inducible nitric oxide synthase (iNOS) is induced in many cell types by cytokines and lipopolysaccharide (LPS). Cytokine signal transduction is believed to be mediated primarily through the JAK/STAT pathway. We therefore examined the effects of a JAK2-specific inhibitor, an antisense oligonucleotide to JAK2, and electroporation of neutralizing anti-STAT1 and anti-STAT3 antibodies on IFNγ- and LPS-stimulated induction of iNOS in vascular smooth muscle cells. Unexpectedly, we found that the JAK/STAT pathway suppresses IFNγ- and LPS-stimulated iNOS induction in these cells. In contrast, the JAK/STAT pathway appears to have a positive role in iNOS induction in RAW 264.7 macrophages.

Original language	English (US)
Pages (from-to)	508-512
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	252
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1998.9678
State	Published - Nov 18 1998

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells",

abstract = "Inducible nitric oxide synthase (iNOS) is induced in many cell types by cytokines and lipopolysaccharide (LPS). Cytokine signal transduction is believed to be mediated primarily through the JAK/STAT pathway. We therefore examined the effects of a JAK2-specific inhibitor, an antisense oligonucleotide to JAK2, and electroporation of neutralizing anti-STAT1 and anti-STAT3 antibodies on IFNγ- and LPS-stimulated induction of iNOS in vascular smooth muscle cells. Unexpectedly, we found that the JAK/STAT pathway suppresses IFNγ- and LPS-stimulated iNOS induction in these cells. In contrast, the JAK/STAT pathway appears to have a positive role in iNOS induction in RAW 264.7 macrophages.",

author = "Marrero, {Mario B.} and Venema, {Virginia J.} and Han He and Caldwell, {Ruth B.} and Venema, {Richard C.}",

note = "Funding Information: This work was supported by National Institutes of Health Grants HL57201 and HL58139, an American Heart Association Grant-in-Aid Award, an American Heart Association/Astra-Merck Grant-in-Aid Award, and an American Heart Association Minority Scientist Developmental Award.",

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doi = "10.1006/bbrc.1998.9678",

language = "English (US)",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells

AU - Marrero, Mario B.

AU - Venema, Virginia J.

AU - He, Han

AU - Caldwell, Ruth B.

AU - Venema, Richard C.

N1 - Funding Information: This work was supported by National Institutes of Health Grants HL57201 and HL58139, an American Heart Association Grant-in-Aid Award, an American Heart Association/Astra-Merck Grant-in-Aid Award, and an American Heart Association Minority Scientist Developmental Award.

PY - 1998/11/18

Y1 - 1998/11/18

N2 - Inducible nitric oxide synthase (iNOS) is induced in many cell types by cytokines and lipopolysaccharide (LPS). Cytokine signal transduction is believed to be mediated primarily through the JAK/STAT pathway. We therefore examined the effects of a JAK2-specific inhibitor, an antisense oligonucleotide to JAK2, and electroporation of neutralizing anti-STAT1 and anti-STAT3 antibodies on IFNγ- and LPS-stimulated induction of iNOS in vascular smooth muscle cells. Unexpectedly, we found that the JAK/STAT pathway suppresses IFNγ- and LPS-stimulated iNOS induction in these cells. In contrast, the JAK/STAT pathway appears to have a positive role in iNOS induction in RAW 264.7 macrophages.

AB - Inducible nitric oxide synthase (iNOS) is induced in many cell types by cytokines and lipopolysaccharide (LPS). Cytokine signal transduction is believed to be mediated primarily through the JAK/STAT pathway. We therefore examined the effects of a JAK2-specific inhibitor, an antisense oligonucleotide to JAK2, and electroporation of neutralizing anti-STAT1 and anti-STAT3 antibodies on IFNγ- and LPS-stimulated induction of iNOS in vascular smooth muscle cells. Unexpectedly, we found that the JAK/STAT pathway suppresses IFNγ- and LPS-stimulated iNOS induction in these cells. In contrast, the JAK/STAT pathway appears to have a positive role in iNOS induction in RAW 264.7 macrophages.

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Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells

Abstract

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