Inhibition of system A amino acid transport activity by ethanol in BeWo choriocarcinoma cells

Chandra R. Jones; Sonne R. Srinivas; Lawrence D. Devoe; Vadivel Ganapathy; Puttur D. Prasad

doi:10.1067/mob.2002.123896

Inhibition of system A amino acid transport activity by ethanol in BeWo choriocarcinoma cells

Chandra R. Jones, Sonne R. Srinivas, Lawrence D. Devoe, Vadivel Ganapathy, Puttur D. Prasad

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

OBJECTIVE: Our purpose was to investigate the influence of ethanol on system A amino acid transporter in BeWo cells. STUDY DESIGN: BeWo cells were cultured in the absence or presence of ethanol. The function of system A was monitored by the transport of α-(methylamino)isobutyric acid. Messenger RNA levels for system A were assessed by Northern analysis. RESULTS: Treatment of BeWo cells with ethanol reduced the activity of system A. The effect was dose and treatment time dependent. The decrease in system A activity was 38% ± 3% at 0.75% ethanol with a 16-hour treatment time. The activities of several other transporters tested were not affected. The effect on system A activity was associated with a decrease in the maximal velocity of the transport system without affecting the substrate affinity. Ethanol did not alter the messenger RNA levels for system A. CONCLUSION: Exposure of BeWo cells to ethanol significantly reduces the function of system A. This finding has potential implications that may be relevant to the pathogenesis of the fetal alcohol syndrome.

Original language	English (US)
Pages (from-to)	209-216
Number of pages	8
Journal	American journal of obstetrics and gynecology
Volume	187
Issue number	1
DOIs	https://doi.org/10.1067/mob.2002.123896
State	Published - Jan 1 2002

Keywords

Alcohol
Amino acid transport
Fetal alcohol syndrome
Intrauterine growth restriction
Pregnancy

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1067/mob.2002.123896

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title = "Inhibition of system A amino acid transport activity by ethanol in BeWo choriocarcinoma cells",

abstract = "OBJECTIVE: Our purpose was to investigate the influence of ethanol on system A amino acid transporter in BeWo cells. STUDY DESIGN: BeWo cells were cultured in the absence or presence of ethanol. The function of system A was monitored by the transport of α-(methylamino)isobutyric acid. Messenger RNA levels for system A were assessed by Northern analysis. RESULTS: Treatment of BeWo cells with ethanol reduced the activity of system A. The effect was dose and treatment time dependent. The decrease in system A activity was 38% ± 3% at 0.75% ethanol with a 16-hour treatment time. The activities of several other transporters tested were not affected. The effect on system A activity was associated with a decrease in the maximal velocity of the transport system without affecting the substrate affinity. Ethanol did not alter the messenger RNA levels for system A. CONCLUSION: Exposure of BeWo cells to ethanol significantly reduces the function of system A. This finding has potential implications that may be relevant to the pathogenesis of the fetal alcohol syndrome.",

keywords = "Alcohol, Amino acid transport, Fetal alcohol syndrome, Intrauterine growth restriction, Pregnancy",

author = "Jones, {Chandra R.} and Srinivas, {Sonne R.} and Devoe, {Lawrence D.} and Vadivel Ganapathy and Prasad, {Puttur D.}",

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AU - Jones, Chandra R.

AU - Srinivas, Sonne R.

AU - Devoe, Lawrence D.

AU - Ganapathy, Vadivel

AU - Prasad, Puttur D.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - OBJECTIVE: Our purpose was to investigate the influence of ethanol on system A amino acid transporter in BeWo cells. STUDY DESIGN: BeWo cells were cultured in the absence or presence of ethanol. The function of system A was monitored by the transport of α-(methylamino)isobutyric acid. Messenger RNA levels for system A were assessed by Northern analysis. RESULTS: Treatment of BeWo cells with ethanol reduced the activity of system A. The effect was dose and treatment time dependent. The decrease in system A activity was 38% ± 3% at 0.75% ethanol with a 16-hour treatment time. The activities of several other transporters tested were not affected. The effect on system A activity was associated with a decrease in the maximal velocity of the transport system without affecting the substrate affinity. Ethanol did not alter the messenger RNA levels for system A. CONCLUSION: Exposure of BeWo cells to ethanol significantly reduces the function of system A. This finding has potential implications that may be relevant to the pathogenesis of the fetal alcohol syndrome.

AB - OBJECTIVE: Our purpose was to investigate the influence of ethanol on system A amino acid transporter in BeWo cells. STUDY DESIGN: BeWo cells were cultured in the absence or presence of ethanol. The function of system A was monitored by the transport of α-(methylamino)isobutyric acid. Messenger RNA levels for system A were assessed by Northern analysis. RESULTS: Treatment of BeWo cells with ethanol reduced the activity of system A. The effect was dose and treatment time dependent. The decrease in system A activity was 38% ± 3% at 0.75% ethanol with a 16-hour treatment time. The activities of several other transporters tested were not affected. The effect on system A activity was associated with a decrease in the maximal velocity of the transport system without affecting the substrate affinity. Ethanol did not alter the messenger RNA levels for system A. CONCLUSION: Exposure of BeWo cells to ethanol significantly reduces the function of system A. This finding has potential implications that may be relevant to the pathogenesis of the fetal alcohol syndrome.

KW - Alcohol

KW - Amino acid transport

KW - Fetal alcohol syndrome

KW - Intrauterine growth restriction

KW - Pregnancy

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Inhibition of system A amino acid transport activity by ethanol in BeWo choriocarcinoma cells

Abstract

Keywords

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