@article{9f6721a9f24243868b0de1bb18ec5ef0,
title = "Inhibition of the G2/M transition of the cell cycle by methyl-2,5-dihydroxycinnamate in human lymphoid cells",
abstract = "Immortalized human lymphoid cells treated with Methyl-2,5-dihydroxycinnamate (MDHC), a stable analog of erbstatin, inhibited the G2/M transition of the cell cycle. The MDHC inhibition of the cell cycle was observed at concentrations well below the IC50 for the inhibition of the EGF receptor and sufficiently below that reported to induce protein cross-linking. The effect of MDHC upon the cell cycle is relatively stable, since unlike erbstatin, inhibition of the G2/M transition was observed 32 hours following removal of the drug. PHA stimulated human peripheral blood mononuclear cells (PBMC) were much less sensitive to MDHC. This study shows that MDHC acts on cells lacking an EGF receptor and the target of MDHC is involved in promoting progression of the cell cycle.",
author = "Koch, {John A.} and Rosenthal, {Ken S.} and Jamison, {James M.} and Summers, {Jack L.}",
note = "Funding Information: Human cell lines studied included a histiocytic lymphoma (U937), and immortalized B-lymphocytes (Daudi) and T-lymphocytes (Jurkat and Hut78). Cells were incubated in RPMI media (10% FCS and penicillin/streptomycin) at 37°C in an atmosphere of 5% CO2. Cells were adjusted to 5 ⨯ 105/ml and incubated in the absence or presence of MDHC (7.7 µM, final concentration) for the indicated times described in the figure legends. Human mononuclear cells were isolated from heparinized blood using Histopaque-1077 (Sigma). The cells were resuspended in RPMI 1This work was supported by a grant from the Summa Health System, Akron, OH 44309. 2To whom correspondence should be addressed. Fax: (330) 325-2524. The abbreviations used are: MDHC, methyl-2,5-dihydroxycinnamate; PHA, phytohemagglutinin; PBS, phosphate buffered saline; FCS, fetal calf serum.",
year = "1996",
month = aug,
day = "14",
doi = "10.1006/bbrc.1996.1207",
language = "English (US)",
volume = "225",
pages = "531--536",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",
}