Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

Sanghamitra Raha; Banabihari Giri; Bhudeb Bhattacharyya; Birendra B. Biswas

doi:10.1016/0014-5793(95)00265-B

Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

Sanghamitra Raha, Banabihari Giri, Bhudeb Bhattacharyya, Birendra B. Biswas

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P₃)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P₄). Similarly, prior depletion of Ins(1,3,4,5)P₄-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P₃. The EC₅₀ for calcium release was 0.15 μM with Ins(1,4,5)P₃ and 0.68 μM with Ins(1,3,4,5)P₄. In conclusion, the Ins(1,3,4,5)P₄-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P₃-sensitive pool.

Original language	English (US)
Pages (from-to)	316-318
Number of pages	3
Journal	FEBS Letters
Volume	362
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)00265-B
State	Published - Apr 10 1995
Externally published	Yes

Keywords

Calcium
Entamoeba histolytica
Fura-2
Inositol(1,3,4,5)tetrakis-phosphate
Inositol(1,4,5)trisphosphate

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)00265-B

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title = "Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica",

abstract = "Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P4). Similarly, prior depletion of Ins(1,3,4,5)P4-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P3. The EC50 for calcium release was 0.15 μM with Ins(1,4,5)P3 and 0.68 μM with Ins(1,3,4,5)P4. In conclusion, the Ins(1,3,4,5)P4-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P3-sensitive pool.",

keywords = "Calcium, Entamoeba histolytica, Fura-2, Inositol(1,3,4,5)tetrakis-phosphate, Inositol(1,4,5)trisphosphate",

author = "Sanghamitra Raha and Banabihari Giri and Bhudeb Bhattacharyya and Biswas, {Birendra B.}",

year = "1995",

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doi = "10.1016/0014-5793(95)00265-B",

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T1 - Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

AU - Raha, Sanghamitra

AU - Giri, Banabihari

AU - Bhattacharyya, Bhudeb

AU - Biswas, Birendra B.

PY - 1995/4/10

Y1 - 1995/4/10

N2 - Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P4). Similarly, prior depletion of Ins(1,3,4,5)P4-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P3. The EC50 for calcium release was 0.15 μM with Ins(1,4,5)P3 and 0.68 μM with Ins(1,3,4,5)P4. In conclusion, the Ins(1,3,4,5)P4-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P3-sensitive pool.

AB - Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P4). Similarly, prior depletion of Ins(1,3,4,5)P4-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P3. The EC50 for calcium release was 0.15 μM with Ins(1,4,5)P3 and 0.68 μM with Ins(1,3,4,5)P4. In conclusion, the Ins(1,3,4,5)P4-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P3-sensitive pool.

KW - Calcium

KW - Entamoeba histolytica

KW - Fura-2

KW - Inositol(1,3,4,5)tetrakis-phosphate

KW - Inositol(1,4,5)trisphosphate

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Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

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