Abstract
The leukocyte integrins LFA-1 and Mac-1 bind to endothelial intercellular adhesion molecule-1 (ICAM-1). Leukocyte adhesion induced by micropipette injection of formylmethionylleucylphenylalanine (fMLP) or macrophage inflammatory protein 2 (MIP-2) next to a venule in the exteriorized mouse cremaster muscle was almost completely blocked after intravenous injection of the ICAM-1 mAb YN-1. In contrast, after 2-h pretreatment with TNF-α, leukocyte adhesion induced in postcapillary venules by fMLP or MIP-2 was not blocked by the ICAM-1 mAb. Leukocyte adhesion was significantly reduced by mAb GAME-46 to CD18 even after TNF-α treatment. We conclude that ICAM-1 is necessary for neutrophil adhesion to unstimulated endothelium, but not for adhesion to cytokine-stimulated endothelium. Although ICAM-1 is expressed at high levels after TNF-α, ICAM-1 either is not functional or is redundant with other endothelial ligands for β2 integrins. (C) 2000 Academic Press.
Original language | English (US) |
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Pages (from-to) | 249-260 |
Number of pages | 12 |
Journal | Microvascular Research |
Volume | 60 |
Issue number | 3 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Adhesion molecule
- Chemokine
- ICAM-1
- LFA-1
- Leukocyte
- MIP-2
- Mac-1
- Mouse
- Venule
- fMLP
ASJC Scopus subject areas
- Biochemistry
- Cardiology and Cardiovascular Medicine
- Cell Biology