Intercellular adhesion molecule-1 is required for chemoattractant-induced leukocyte adhesion in resting, but not inflamed, venules in vivo

Daniel S. Foy, Klaus Ley

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The leukocyte integrins LFA-1 and Mac-1 bind to endothelial intercellular adhesion molecule-1 (ICAM-1). Leukocyte adhesion induced by micropipette injection of formylmethionylleucylphenylalanine (fMLP) or macrophage inflammatory protein 2 (MIP-2) next to a venule in the exteriorized mouse cremaster muscle was almost completely blocked after intravenous injection of the ICAM-1 mAb YN-1. In contrast, after 2-h pretreatment with TNF-α, leukocyte adhesion induced in postcapillary venules by fMLP or MIP-2 was not blocked by the ICAM-1 mAb. Leukocyte adhesion was significantly reduced by mAb GAME-46 to CD18 even after TNF-α treatment. We conclude that ICAM-1 is necessary for neutrophil adhesion to unstimulated endothelium, but not for adhesion to cytokine-stimulated endothelium. Although ICAM-1 is expressed at high levels after TNF-α, ICAM-1 either is not functional or is redundant with other endothelial ligands for β2 integrins. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)249-260
Number of pages12
JournalMicrovascular Research
Volume60
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Adhesion molecule
  • Chemokine
  • ICAM-1
  • LFA-1
  • Leukocyte
  • MIP-2
  • Mac-1
  • Mouse
  • Venule
  • fMLP

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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