Intermittent fasting attenuates inflammasome activity in ischemic stroke

David Yang Wei Fann; Tomislav Santro; Silvia Manzanero; Alexander Widiapradja; Yi Lin Cheng; Seung Yoon Lee; Prasad Chunduri; Dong Gyu Jo; Alexis M. Stranahan; Mark P. Mattson; Thiruma V. Arumugam

doi:10.1016/j.expneurol.2014.04.017

Intermittent fasting attenuates inflammasome activity in ischemic stroke

David Yang Wei Fann, Tomislav Santro, Silvia Manzanero, Alexander Widiapradja, Yi Lin Cheng, Seung Yoon Lee, Prasad Chunduri, Dong Gyu Jo, Alexis M. Stranahan, Mark P. Mattson, Thiruma V. Arumugam

Neuroscience and Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16. h of food deprivation daily) for 4. months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.

Original language	English (US)
Pages (from-to)	114-119
Number of pages	6
Journal	Experimental Neurology
Volume	257
DOIs	https://doi.org/10.1016/j.expneurol.2014.04.017
State	Published - Jul 2014

Keywords

Inflammasomes
Intermittent fasting
Ischemic stroke
NLRP1
NLRP3

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/j.expneurol.2014.04.017

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title = "Intermittent fasting attenuates inflammasome activity in ischemic stroke",

abstract = "Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16. h of food deprivation daily) for 4. months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.",

keywords = "Inflammasomes, Intermittent fasting, Ischemic stroke, NLRP1, NLRP3",

author = "Fann, {David Yang Wei} and Tomislav Santro and Silvia Manzanero and Alexander Widiapradja and Cheng, {Yi Lin} and Lee, {Seung Yoon} and Prasad Chunduri and Jo, {Dong Gyu} and Stranahan, {Alexis M.} and Mattson, {Mark P.} and Arumugam, {Thiruma V.}",

note = "Funding Information: The ARC Future Fellowship ( FT100100427 ) awarded to TVA, and the National Institute on Aging Intramural Research Program supported this work.",

year = "2014",

month = jul,

doi = "10.1016/j.expneurol.2014.04.017",

language = "English (US)",

volume = "257",

pages = "114--119",

journal = "Experimental Neurology",

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AU - Fann, David Yang Wei

AU - Santro, Tomislav

AU - Manzanero, Silvia

AU - Widiapradja, Alexander

AU - Cheng, Yi Lin

AU - Lee, Seung Yoon

AU - Chunduri, Prasad

AU - Jo, Dong Gyu

AU - Stranahan, Alexis M.

AU - Mattson, Mark P.

AU - Arumugam, Thiruma V.

N1 - Funding Information: The ARC Future Fellowship ( FT100100427 ) awarded to TVA, and the National Institute on Aging Intramural Research Program supported this work.

PY - 2014/7

Y1 - 2014/7

N2 - Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16. h of food deprivation daily) for 4. months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.

AB - Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16. h of food deprivation daily) for 4. months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.

KW - Inflammasomes

KW - Intermittent fasting

KW - Ischemic stroke

KW - NLRP1

KW - NLRP3

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Intermittent fasting attenuates inflammasome activity in ischemic stroke

Abstract

Keywords

ASJC Scopus subject areas

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