Abstract
MRI has been used to evaluate labeled cell migration and distribution. However, quantitative determination of labeled cell concentration using MRI has not been systematically investigated. In the current study, we investigated the relationships between labeled cell concentration and MRI parameters of transverse relaxation rate, R2, and apparent diffusion coefficient (ADC), in vitro in phantoms and in vivo in rats after stroke. Significant correlations were detected between iron concentration or labeled cell concentration and MRI measurements of R2, ADC, and ADCxR2 in vitro. In contrast, in vivo labeled cell concentration did not significantly correlate with R2, ADC, and ADCxR2. A major factor for the absence of a significant correlation between labeled cell concentration and MRI measurements in vivo may be attributed to background effects of ischemic tissue. By correcting the background effects caused by ischemic damage, ΔR2 (difference in R2 values in the ischemic tissue with and without labeled cells) exhibited a significant correlation to labeled cell concentration. Our study suggests that MRI parameters have the potential to quantitatively determine labeled cell concentration in vivo.
Original language | English (US) |
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Pages (from-to) | 587-594 |
Number of pages | 8 |
Journal | Magnetic Resonance in Medicine |
Volume | 61 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2009 |
Externally published | Yes |
Keywords
- Diffusion coefficient
- MRI labeled cells
- Magnetic resonance imaging
- Molecular imaging
- Stroke
- T
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging