@article{012e71a0ef034751bd1a6351eb614c85,
title = "Investigation of the effect of dietary intake of omega-3 polyunsaturated fatty acids on trauma-induced white matter injury with quantitative diffusion MRI in mice",
abstract = "Previous studies suggest that long-term supplementation and dietary intake of omega-3 polyunsaturated fatty acids (PUFAs) may have neuroprotective effects following brain injury. The objective of this study was to investigate potential neuroprotective effects of omega-3 PUFAs on white matter following closed-head trauma. The closed-head injury model of engineered rotational acceleration (CHIMERA) produces a reproducible injury in the optic tract and brachium of the superior colliculus in mice. Damage is detectable using diffusion tensor imaging (DTI) metrics, particularly fractional anisotropy (FA), with sensitivity comparable to histology. We acquired in vivo (n = 38) and ex vivo (n = 41) DTI data in mice divided into sham and CHIMERA groups with two dietary groups: one deficient in omega-3 PUFAs and one adequate in omega-3 PUFAs. We examined injury effects (reduction in FA) and neuroprotection (FA reduction modulated by diet) in the optic tract and brachium. We verified that diet did not affect FA in sham animals. In injured animals, we found significantly reduced FA in the optic tract and brachium (~10% reduction, p < 0.001), and Bayes factor analysis showed strong evidence to reject the null hypothesis. However, Bayes factor analysis showed substantial evidence to accept the null hypothesis of no diet-related FA differences in injured animals in the in vivo and ex vivo samples. Our results indicate no neuroprotective effect from adequate dietary omega-3 PUFA intake on white matter damage following traumatic brain injury. Since damage from CHIMERA mainly affects white matter, our results do not necessarily contradict previous findings showing omega-3 PUFA-mediated neuroprotection in gray matter.",
keywords = "MRI, closed-head injury, diffusion tensor imaging, mouse, omega-3 fatty acids, traumatic brain injury",
author = "Reyes, {Laura D.} and Thaddeus Haight and Abhishek Desai and Huazhen Chen and Asamoah Bosomtwi and Alexandru Korotcov and Bernard Dardzinski and Kim, {Hee Yong} and Carlo Pierpaoli",
note = "Funding Information: The authors thank Dr. Cheryl Wellington and her group in the Department of Pathology and Laboratory Medicine, Djavad Mowafaghian Centre for Brain Health, University of British Columbia for the CHIMERA device and training. The authors thank Dr. Elizabeth Hutchinson, Dr. Neda Sadeghi, Amritha Nayak, and Dr. Okan Irfanoglu for their input and advice on image processing and analyses throughout the project. Support for this project was provided by CNRM HJF Award Number 307513‐3.01‐60855. This research was supported in part by the Intramural Research Program of the NIH, including the National Institute of Biomedical Imaging and Bioengineering and the National Institute on Alcohol Abuse and Alcoholism. The opinions and assertions expressed herein are those of the authors and do not necessarily reflect the official policy or position of the Uniformed Services University, the Department of Defense, the U.S. Government, or the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. Funding Information: CNRM HJF Award Number 307513-3.01-60855 The authors thank Dr. Cheryl Wellington and her group in the Department of Pathology and Laboratory Medicine, Djavad Mowafaghian Centre for Brain Health, University of British Columbia for the CHIMERA device and training. The authors thank Dr. Elizabeth Hutchinson, Dr. Neda Sadeghi, Amritha Nayak, and Dr. Okan Irfanoglu for their input and advice on image processing and analyses throughout the project. Support for this project was provided by CNRM HJF Award Number 307513-3.01-60855. This research was supported in part by the Intramural Research Program of the NIH, including the National Institute of Biomedical Imaging and Bioengineering and the National Institute on Alcohol Abuse and Alcoholism. The opinions and assertions expressed herein are those of the authors and do not necessarily reflect the official policy or position of the Uniformed Services University, the Department of Defense, the U.S. Government, or the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. Publisher Copyright: {\textcopyright} 2020 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC.",
year = "2020",
month = nov,
day = "1",
doi = "10.1002/jnr.24705",
language = "English (US)",
volume = "98",
pages = "2232--2244",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "11",
}