TY - JOUR
T1 - Ion chromatographic method for the analysis of sulfate in complex carbohydrates
AU - Grotjan, H. Edward
AU - Padrnos-Hicks, Paula A.
AU - Keel, Brooks A.
N1 - Funding Information:
Tom Jupille, Dave Togami and Pat Barthel of WescanI nstrumentsa s well as Dr. Loui Silvestri provided helpful suggestionsP. aula A. Padrnos-Hicks was supported by a Bush Foundation Grant from the University of South Dakota. Other supportw as from NIH Grant HD18879a s well as the Parson’sE ndowmentt hrough the University of South Dakota School of Medicine.
PY - 1986
Y1 - 1986
N2 - A procedure is described for the quantitation of sulfate in complex carbohydrates. Samples are taken to dryness in the presence of a small amount of sodium hydroxide and the sulfate is liberated by pyrolysis. Sulfate is then quantitated by single-column ion chromatography eliminating the interference by ions such as phosphate, chloride, sodium and calcium which frequently occurs with colorimetric methods. The reproducibility of the method is approximately 10%. This approach cannot be directly applied to glycoproteins because a portion of the sulfur in sulfur-containing amino acids appears to be converted to sulfate. However, this method can be used to measure sulfate in oligosaccharides of glycoproteins if the protein components are removed prior to analysis.
AB - A procedure is described for the quantitation of sulfate in complex carbohydrates. Samples are taken to dryness in the presence of a small amount of sodium hydroxide and the sulfate is liberated by pyrolysis. Sulfate is then quantitated by single-column ion chromatography eliminating the interference by ions such as phosphate, chloride, sodium and calcium which frequently occurs with colorimetric methods. The reproducibility of the method is approximately 10%. This approach cannot be directly applied to glycoproteins because a portion of the sulfur in sulfur-containing amino acids appears to be converted to sulfate. However, this method can be used to measure sulfate in oligosaccharides of glycoproteins if the protein components are removed prior to analysis.
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U2 - 10.1016/S0021-9673(00)94857-X
DO - 10.1016/S0021-9673(00)94857-X
M3 - Article
C2 - 3782351
AN - SCOPUS:0022535371
SN - 0021-9673
VL - 367
SP - 367
EP - 375
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -