Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

Toshio Ariga; Michiko Sekine; Robert K. Yu; Tadashi Miyatake

doi:10.1016/0003-9861(84)90546-0

Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

Toshio Ariga, Michiko Sekine, Robert K. Yu, Tadashi Miyatake

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A novel G_Dlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as G_Dlb (NeuGc)₂ or II³ (NeuGc)₂-GgOse₄Cer.

Original language	English (US)
Pages (from-to)	305-309
Number of pages	5
Journal	Archives of Biochemistry and Biophysics
Volume	232
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(84)90546-0
State	Published - Jul 1984
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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@article{0667ccaf87c44d3facb74dd0bae19f4a,

title = "Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla",

abstract = "A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.",

author = "Toshio Ariga and Michiko Sekine and Yu, {Robert K.} and Tadashi Miyatake",

note = "Funding Information: This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, and Culture of Japan. R.K.Y. was supported by USPHS Grant NS 11853 and NMSS Grant RG 1289-B-3. We thank Ms. Debbie Tewksbury for careful and prompt word processing.",

year = "1984",

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doi = "10.1016/0003-9861(84)90546-0",

language = "English (US)",

volume = "232",

pages = "305--309",

journal = "Archives of Biochemistry and Biophysics",

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number = "1",

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TY - JOUR

T1 - Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

AU - Ariga, Toshio

AU - Sekine, Michiko

AU - Yu, Robert K.

AU - Miyatake, Tadashi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, and Culture of Japan. R.K.Y. was supported by USPHS Grant NS 11853 and NMSS Grant RG 1289-B-3. We thank Ms. Debbie Tewksbury for careful and prompt word processing.

PY - 1984/7

Y1 - 1984/7

N2 - A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.

AB - A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.

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Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

Abstract

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