Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk

Daniela M. Bonafede, Lawrence J. Macala, Martha Costantine-Paton, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.

Original languageEnglish (US)
Pages (from-to)680-684
Number of pages5
Issue number8
StatePublished - Aug 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


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