Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk

Daniela M. Bonafede; Lawrence J. Macala; Martha Costantine-Paton; Robert K. Yu

doi:10.1007/BF02535204

Isolation and characterization of ganglioside 9-O-acetyl-GD₃ from bovine buttermilk

Daniela M. Bonafede, Lawrence J. Macala, Martha Costantine-Paton, Robert K. Yu

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41 Scopus citations

Abstract

Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD₃. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD₃ was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD₃ by mild base treatment.

Original language	English (US)
Pages (from-to)	680-684
Number of pages	5
Journal	Lipids
Volume	24
Issue number	8
DOIs	https://doi.org/10.1007/BF02535204
State	Published - Aug 1989
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

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abstract = "Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.",

author = "Bonafede, {Daniela M.} and Macala, {Lawrence J.} and Martha Costantine-Paton and Yu, {Robert K.}",

year = "1989",

month = aug,

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pages = "680--684",

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T1 - Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk

AU - Bonafede, Daniela M.

AU - Macala, Lawrence J.

AU - Costantine-Paton, Martha

AU - Yu, Robert K.

PY - 1989/8

Y1 - 1989/8

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AB - Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.

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IS - 8

ER -

Isolation and characterization of ganglioside 9-O-acetyl-GD₃ from bovine buttermilk

Abstract

ASJC Scopus subject areas

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