TY - JOUR
T1 - Label-free detection of proteins in ternary mixtures using surface-enhanced Raman scattering and protein melting profiles
AU - Keskin, Sercan
AU - Efeoǧlu, Esen
AU - Keçeci, Kaan
AU - Çulha, Mustafa
N1 - Funding Information:
The authors acknowledge the financial support of the Scientific and Technological Council of Turkey (Project no: 109T941) and Yeditepe University.
PY - 2013/3
Y1 - 2013/3
N2 - The multiplex detection of biologically important molecules such as proteins in complex mixtures has critical importance not only in disease diagnosis but also in other fields such as proteomics and biotechnology. Surface-enhanced Raman scattering (SERS) is a powerful technique for multiplex identification of molecular components in a mixture. We combined the multiplexing power of SERS and heat denaturation of proteins to identify proteins in ternary protein mixtures. The heat denaturation profiles of four model blood proteins, transferrin, human serum albumin, fibrinogen, and hemoglobin, were studied with SERS. Then, two ternary mixtures of these four proteins were used to test the feasibility of the approach. It was demonstrated that unique denaturation profiles of each protein could be used for their identification in the mixture.
AB - The multiplex detection of biologically important molecules such as proteins in complex mixtures has critical importance not only in disease diagnosis but also in other fields such as proteomics and biotechnology. Surface-enhanced Raman scattering (SERS) is a powerful technique for multiplex identification of molecular components in a mixture. We combined the multiplexing power of SERS and heat denaturation of proteins to identify proteins in ternary protein mixtures. The heat denaturation profiles of four model blood proteins, transferrin, human serum albumin, fibrinogen, and hemoglobin, were studied with SERS. Then, two ternary mixtures of these four proteins were used to test the feasibility of the approach. It was demonstrated that unique denaturation profiles of each protein could be used for their identification in the mixture.
KW - Heat denaturation
KW - Multiplex detection
KW - Protein
KW - surface-enhanced Raman scattering
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U2 - 10.1117/1.JBO.18.3.037007
DO - 10.1117/1.JBO.18.3.037007
M3 - Article
C2 - 23515867
AN - SCOPUS:84886046602
SN - 1083-3668
VL - 18
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 3
M1 - 037007
ER -