TY - JOUR
T1 - Large and small tumor antigens from simian virus 40 have identical amino termini mapping at 0.65 map units
AU - Paucha, E.
AU - Mellor, A.
AU - Harvey, R.
AU - Smith, A. E.
AU - Hewick, R. M.
AU - Waterfield, M. D.
PY - 1978
Y1 - 1978
N2 - Large and small tumor (T) antigens of simian virus 40 were synthesized in vitro with L-cell extracts that had been treated by the method of Palmiter to prevent amino-terminal acetylation of nascent proteins. Partial amino-terminal amino acid sequences of both forms of T-antigen were determined and found to be identical. Methionine residues were located at positions 1 and 14, a lysine residue at position 3, and leucine residues at positions 5, 11, 13, 16, 17, and 19. These amino acid sequence data match perfectly the amino acid sequence predicted from a sequence of nucleotides in the E strand of simian virus 40 DNA which begins near the junction between HindII/III fragments A and C at about 0.65 map units. This strongly suggests that the sequence coding for the amino terminus of both proteins is located at this position. Furthermore, the data are consistent with a model for the synthesis of both forms of T-antigen that predicts that: small T-antigen is coded for by a sequence of nucleotides from the 5' end of the early region; and large T-antigen is coded for by nucleotide sequences from two noncontiguous regions of simian virus 40 DNA.
AB - Large and small tumor (T) antigens of simian virus 40 were synthesized in vitro with L-cell extracts that had been treated by the method of Palmiter to prevent amino-terminal acetylation of nascent proteins. Partial amino-terminal amino acid sequences of both forms of T-antigen were determined and found to be identical. Methionine residues were located at positions 1 and 14, a lysine residue at position 3, and leucine residues at positions 5, 11, 13, 16, 17, and 19. These amino acid sequence data match perfectly the amino acid sequence predicted from a sequence of nucleotides in the E strand of simian virus 40 DNA which begins near the junction between HindII/III fragments A and C at about 0.65 map units. This strongly suggests that the sequence coding for the amino terminus of both proteins is located at this position. Furthermore, the data are consistent with a model for the synthesis of both forms of T-antigen that predicts that: small T-antigen is coded for by a sequence of nucleotides from the 5' end of the early region; and large T-antigen is coded for by nucleotide sequences from two noncontiguous regions of simian virus 40 DNA.
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U2 - 10.1073/pnas.75.5.2165
DO - 10.1073/pnas.75.5.2165
M3 - Article
C2 - 209456
AN - SCOPUS:0018143530
SN - 0003-1348
VL - 75
SP - 2165
EP - 2169
JO - Unknown Journal
JF - Unknown Journal
IS - 5
ER -