Liberation of hydroxyproline from gelatin by acid hydrolysis

David H. Pashley, C. Keith Claycomb, Gordon W. Summers

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

Original languageEnglish (US)
Pages (from-to)154-156
Number of pages3
JournalAnalytical Biochemistry
Issue number1
StatePublished - Apr 1966
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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