Liberation of hydroxyproline from gelatin by acid hydrolysis

David H. Pashley; C. Keith Claycomb; Gordon W. Summers

doi:10.1016/0003-2697(66)90258-2

Liberation of hydroxyproline from gelatin by acid hydrolysis

David H. Pashley
, C. Keith Claycomb
, Gordon W. Summers

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

Original language	English (US)
Pages (from-to)	154-156
Number of pages	3
Journal	Analytical Biochemistry
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(66)90258-2
State	Published - Apr 1966
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(66)90258-2

Cite this

@article{9cecce1ebb44452d9a069bdf1309e38f,

title = "Liberation of hydroxyproline from gelatin by acid hydrolysis",

abstract = "A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.",

author = "Pashley, \{David H.\} and Claycomb, \{C. Keith\} and Summers, \{Gordon W.\}",

note = "Funding Information: The authors gratefully acknowledge the assistance of S. Robert \&how. This investigation was supported in part by the United States Public Health Service Research Grant DE 01908-02 from the National Institut\textasciitilde{}c of Dental Research, National Institutes of Health, Bethesda, Maryland.",

year = "1966",

month = apr,

doi = "10.1016/0003-2697(66)90258-2",

language = "English (US)",

volume = "15",

pages = "154--156",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Liberation of hydroxyproline from gelatin by acid hydrolysis

AU - Pashley, David H.

AU - Claycomb, C. Keith

AU - Summers, Gordon W.

N1 - Funding Information: The authors gratefully acknowledge the assistance of S. Robert &how. This investigation was supported in part by the United States Public Health Service Research Grant DE 01908-02 from the National Institut~c of Dental Research, National Institutes of Health, Bethesda, Maryland.

PY - 1966/4

Y1 - 1966/4

N2 - A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

AB - A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

UR - https://www.scopus.com/pages/publications/0013896084

UR - https://www.scopus.com/pages/publications/0013896084#tab=citedBy

U2 - 10.1016/0003-2697(66)90258-2

DO - 10.1016/0003-2697(66)90258-2

M3 - Article

C2 - 5959419

AN - SCOPUS:0013896084

SN - 0003-2697

VL - 15

SP - 154

EP - 156

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Liberation of hydroxyproline from gelatin by acid hydrolysis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this