TY - JOUR
T1 - Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis
AU - McArdle, Sara
AU - Mikulski, Zbigniew
AU - Ley, Klaus
N1 - Publisher Copyright:
© 2016 McArdle et al.
PY - 2016/6/27
Y1 - 2016/6/27
N2 - Intravital imaging is an invaluable tool for understanding the function of cells in healthy and diseased tissues. It provides a window into dynamic processes that cannot be studied by other techniques. This review will cover the benefits and limitations of various techniques for labeling and imaging myeloid cells, with a special focus on imaging cells in atherosclerotic arteries. Although intravital imaging is a powerful tool for understanding cell function, it alone does not provide a complete picture of the cell. Other techniques, such as flow cytometry and transcriptomics, must be combined with intravital imaging to fully understand a cell's phenotype, lineage, and function.
AB - Intravital imaging is an invaluable tool for understanding the function of cells in healthy and diseased tissues. It provides a window into dynamic processes that cannot be studied by other techniques. This review will cover the benefits and limitations of various techniques for labeling and imaging myeloid cells, with a special focus on imaging cells in atherosclerotic arteries. Although intravital imaging is a powerful tool for understanding cell function, it alone does not provide a complete picture of the cell. Other techniques, such as flow cytometry and transcriptomics, must be combined with intravital imaging to fully understand a cell's phenotype, lineage, and function.
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U2 - 10.1084/jem.20151885
DO - 10.1084/jem.20151885
M3 - Article
C2 - 27270892
AN - SCOPUS:84977641150
SN - 0022-1007
VL - 213
SP - 1117
EP - 1131
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 7
ER -