TY - JOUR
T1 - Long-term sustainable dendritic cell-specific depletion murine model for periodontitis research
AU - Finger Stadler, Amanda
AU - Patel, Mitulkumar
AU - Pacholczyk, Rafal
AU - Cutler, Christopher W.
AU - Arce, Roger M.
N1 - Funding Information:
Funding from National Institutes of Health (NIH/NIDCR R01 DE014328, to CWC) and the Robert Wood Johnson Foundation - Harold Amos Medical Faculty Development Program (ID 38138, to RMA) partially supported this study. The RWJF had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/10
Y1 - 2017/10
N2 - Dendritic cells (DCs) are specialized antigen-presenting cells that play a pivotal role in the pathogenesis of periodontitis. The use of animal models to study the role of DCs in periodontitis has been limited by lack of a method for sustained depletion of DCs. Hence, the objectives of this study were to validate the zDC-DTR knockin mouse model of conventional DCs (cDCs) depletion, as well as to investigate whether this depletion could be sustained long enough to induce alveolar bone loss in this model. zDC-DTR mice were treated with different dose regimens of diphtheria toxin (DT) to determine survival rate. A loading DT dose of 20 ng/bw, followed and maintained with doses of 10 ng/bm every 3 days for up to 4 weeks demonstrated 80% survival. Animals were weighed weekly and peripheral blood was obtained to confirm normal neutrophil counts. Five animals per group were euthanized at baseline, 24 h, 1 and 4 weeks. Bone marrow (BM), spleen (SP) and gingival tissue (GT) were harvested, and cells were isolated, separated and stained for Pre-DCs precursors (CD45R− MHCII+ CD11c+ Flt3+ CD172a+) in BM, cDCs (CD11c+ MHCII+ CD209+) in spleen, and DCs in GT (CD45R+ MHCII+ CD11c+ DC-SIGN/CD209+). Pre-DCs in BM were significantly depleted at 24 h and depletion maintained for up to 4 weeks, as compared to blank (PBS) controls. Circulating cDCs in spleen demonstrated a non-significant trend to deplete in 1 week with high variability among mice. GT also showed a similar non-significant trend to deplete in 24 h. The zDC-DTR model seems to be viable for evaluating the role of DCs immune homeostasis disruption and alveolar bone loss pathogenesis in response to long-term oral infection.
AB - Dendritic cells (DCs) are specialized antigen-presenting cells that play a pivotal role in the pathogenesis of periodontitis. The use of animal models to study the role of DCs in periodontitis has been limited by lack of a method for sustained depletion of DCs. Hence, the objectives of this study were to validate the zDC-DTR knockin mouse model of conventional DCs (cDCs) depletion, as well as to investigate whether this depletion could be sustained long enough to induce alveolar bone loss in this model. zDC-DTR mice were treated with different dose regimens of diphtheria toxin (DT) to determine survival rate. A loading DT dose of 20 ng/bw, followed and maintained with doses of 10 ng/bm every 3 days for up to 4 weeks demonstrated 80% survival. Animals were weighed weekly and peripheral blood was obtained to confirm normal neutrophil counts. Five animals per group were euthanized at baseline, 24 h, 1 and 4 weeks. Bone marrow (BM), spleen (SP) and gingival tissue (GT) were harvested, and cells were isolated, separated and stained for Pre-DCs precursors (CD45R− MHCII+ CD11c+ Flt3+ CD172a+) in BM, cDCs (CD11c+ MHCII+ CD209+) in spleen, and DCs in GT (CD45R+ MHCII+ CD11c+ DC-SIGN/CD209+). Pre-DCs in BM were significantly depleted at 24 h and depletion maintained for up to 4 weeks, as compared to blank (PBS) controls. Circulating cDCs in spleen demonstrated a non-significant trend to deplete in 1 week with high variability among mice. GT also showed a similar non-significant trend to deplete in 24 h. The zDC-DTR model seems to be viable for evaluating the role of DCs immune homeostasis disruption and alveolar bone loss pathogenesis in response to long-term oral infection.
KW - Animal models
KW - Dendritic cells
KW - Diphtheria toxin
KW - Knockout
KW - Mice
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U2 - 10.1016/j.jim.2017.06.007
DO - 10.1016/j.jim.2017.06.007
M3 - Article
C2 - 28645528
AN - SCOPUS:85021200024
SN - 0022-1759
VL - 449
SP - 7
EP - 14
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -