Low access resistance perforated patch recordings using amphotericin B

James Rae; Kim Cooper; Peter Gates; Mitchell Watsky

doi:10.1016/0165-0270(91)90017-T

Low access resistance perforated patch recordings using amphotericin B

James Rae, Kim Cooper, Peter Gates, Mitchell Watsky

Research output: Contribution to journal › Article › peer-review

164 Scopus citations

Abstract

We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

Original language	English (US)
Pages (from-to)	15-26
Number of pages	12
Journal	Journal of Neuroscience Methods
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0165-0270(91)90017-T
State	Published - Mar 1991
Externally published	Yes

Keywords

Amphotericin
Nystatin
Patch clamp
Perforated patch
Resting voltage
Single channels
Whole cell currents

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0165-0270(91)90017-T

Cite this

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title = "Low access resistance perforated patch recordings using amphotericin B",

abstract = "We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new {"}outside-out patch{"} preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.",

keywords = "Amphotericin, Nystatin, Patch clamp, Perforated patch, Resting voltage, Single channels, Whole cell currents",

author = "James Rae and Kim Cooper and Peter Gates and Mitchell Watsky",

note = "Funding Information: This work was supported by NIH grants EY03282, EY06005, EY06206, and an unrestricted award from Research to Prevent Blindness. We thank Dick Horn for sharing his nystatin techniques with us and for discussions about using perforated patches for outside-out single channel recordings.",

year = "1991",

month = mar,

doi = "10.1016/0165-0270(91)90017-T",

language = "English (US)",

volume = "37",

pages = "15--26",

journal = "Journal of Neuroscience Methods",

issn = "0165-0270",

publisher = "Elsevier",

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TY - JOUR

T1 - Low access resistance perforated patch recordings using amphotericin B

AU - Rae, James

AU - Cooper, Kim

AU - Gates, Peter

AU - Watsky, Mitchell

N1 - Funding Information: This work was supported by NIH grants EY03282, EY06005, EY06206, and an unrestricted award from Research to Prevent Blindness. We thank Dick Horn for sharing his nystatin techniques with us and for discussions about using perforated patches for outside-out single channel recordings.

PY - 1991/3

Y1 - 1991/3

N2 - We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

AB - We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

KW - Amphotericin

KW - Nystatin

KW - Patch clamp

KW - Perforated patch

KW - Resting voltage

KW - Single channels

KW - Whole cell currents

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JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 1

ER -

Low access resistance perforated patch recordings using amphotericin B

Abstract

Keywords

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