Low access resistance perforated patch recordings using amphotericin B

James Rae, Kim Cooper, Peter Gates, Mitchell Watsky

Research output: Contribution to journalArticlepeer-review

164 Scopus citations


We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalJournal of Neuroscience Methods
Issue number1
StatePublished - Mar 1991
Externally publishedYes


  • Amphotericin
  • Nystatin
  • Patch clamp
  • Perforated patch
  • Resting voltage
  • Single channels
  • Whole cell currents

ASJC Scopus subject areas

  • Neuroscience(all)


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