TY - JOUR
T1 - Lupeol and its ester ameliorate the cyclophosphamide provoked cardiac lysosomal damage studied in rat
AU - Sudharsan, Periyasamy Thandavan
AU - Mythili, Yenjerla
AU - Selvakumar, Elangovan
AU - Varalakshmi, Palaninathan
N1 - Funding Information:
We thank the Indian Council of Medical Research (ICMR), Government of India, New Delhi, for providing the financial assistance in the form of Senior Research Fellowship to the first and third author, and Junior Research Fellowship to the second author. We thank Dr Nachiappa Ganesh Rajesh, M.D. (Path), for his valuable help in interpreting the transmission electron microscopy specimens.
PY - 2006/1
Y1 - 2006/1
N2 - Cyclophosphamide (CP), an alkylating agent widely used in cancer chemotherapy causes fatal cardiotoxicity. Lupeol, a pentacyclic triterpene, isolated from Crataeva nurvala stem bark and its ester, lupeol linoleate possess a wide range of medicinal properties. The effect of lupeol and its ester was evaluated in CP-induced myocardial toxicity in rats. Male albino rats of Wistar strain were categorized into six groups. Group I served as control. Rats in groups II, V and VI animals were injected intraperitoneally with a single dose of CP (200 mg/ kg body weight) dissolved in saline. CP-treated groups V and VI received lupeol and lupeol linoleate (50 mg/kg body weight), respectively, dissolved in olive oil for 10 days by oral gavage. CP-administered rats showed a significant increase (p < 0.001) in the activities of lysosomal hydrolases in serum and heart, a decrease (p < 0.001) in the levels of cellular thiols and myofibres were swollen with loss of myofilaments in electron microscopical analysis in heart. Lupeol and its ester showed reversal of the above alterations induced by CP. These findings demonstrate that the supplementation with lupeol and its ester could preserve lysosomal integrity, improve thiol levels, highlighting their protective effect against CP-induced cardiotoxicity.
AB - Cyclophosphamide (CP), an alkylating agent widely used in cancer chemotherapy causes fatal cardiotoxicity. Lupeol, a pentacyclic triterpene, isolated from Crataeva nurvala stem bark and its ester, lupeol linoleate possess a wide range of medicinal properties. The effect of lupeol and its ester was evaluated in CP-induced myocardial toxicity in rats. Male albino rats of Wistar strain were categorized into six groups. Group I served as control. Rats in groups II, V and VI animals were injected intraperitoneally with a single dose of CP (200 mg/ kg body weight) dissolved in saline. CP-treated groups V and VI received lupeol and lupeol linoleate (50 mg/kg body weight), respectively, dissolved in olive oil for 10 days by oral gavage. CP-administered rats showed a significant increase (p < 0.001) in the activities of lysosomal hydrolases in serum and heart, a decrease (p < 0.001) in the levels of cellular thiols and myofibres were swollen with loss of myofilaments in electron microscopical analysis in heart. Lupeol and its ester showed reversal of the above alterations induced by CP. These findings demonstrate that the supplementation with lupeol and its ester could preserve lysosomal integrity, improve thiol levels, highlighting their protective effect against CP-induced cardiotoxicity.
KW - Cyclophosphamide
KW - Electron microscopy
KW - Lupeol
KW - Lupeol linoleate
KW - Lysosomal destabilization
KW - Thiols
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U2 - 10.1007/s11010-006-1169-1
DO - 10.1007/s11010-006-1169-1
M3 - Article
C2 - 16317508
AN - SCOPUS:29644435334
SN - 0300-8177
VL - 282
SP - 23
EP - 29
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
IS - 1-2
ER -
