TY - JOUR
T1 - Measurement of the Membrane Potential of Isolated Nerve Terminals by the Lipophilic Cation [3H]Triphenylmethylphosphonium Bromide
AU - Hansson, Elisabeth
AU - Jacobson, Ingemar
AU - Venema, Richard
AU - Sellström, Åke
PY - 1980/3
Y1 - 1980/3
N2 - Abstract: The lipophilic cation [3H]triphenylrnethylphosphonium bromide ([3H]TPMP+) was investigated as a measure of the membrane potential of synaptosomes. Conditions under which [3H]TPMP+ achieved an equilibrium distribution were tested. The toxicity of TPMP has been studied relative to its inhibitory effects on [3H]y‐aminobutyric acid ([3H]GABA) transport. In some experiments the distribution of 86RbZ+ and [3H]TPMP+ was changed upon incubation in the presence of elevated levels of K+, ouabain, or KCN, or at 0°C in a way that would be expected from the membrane potential. In normal incubation conditions a membrane potential of ∼−60 mv was calculated.
AB - Abstract: The lipophilic cation [3H]triphenylrnethylphosphonium bromide ([3H]TPMP+) was investigated as a measure of the membrane potential of synaptosomes. Conditions under which [3H]TPMP+ achieved an equilibrium distribution were tested. The toxicity of TPMP has been studied relative to its inhibitory effects on [3H]y‐aminobutyric acid ([3H]GABA) transport. In some experiments the distribution of 86RbZ+ and [3H]TPMP+ was changed upon incubation in the presence of elevated levels of K+, ouabain, or KCN, or at 0°C in a way that would be expected from the membrane potential. In normal incubation conditions a membrane potential of ∼−60 mv was calculated.
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U2 - 10.1111/j.1471-4159.1980.tb11182.x
DO - 10.1111/j.1471-4159.1980.tb11182.x
M3 - Article
C2 - 7354332
AN - SCOPUS:0018901502
SN - 0022-3042
VL - 34
SP - 569
EP - 573
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 3
ER -