Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes

B. M. Sutherland; J. C. Sutherland

doi:10.1016/S0006-3495(69)86387-3

Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes

B. M. Sutherland, J. C. Sutherland

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33 Scopus citations

Abstract

The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S₁) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.

Original language	English (US)
Pages (from-to)	292-302
Number of pages	11
Journal	Biophysical Journal
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/S0006-3495(69)86387-3
State	Published - 1969
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(69)86387-3

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title = "Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes",

abstract = "The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S1) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.",

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year = "1969",

doi = "10.1016/S0006-3495(69)86387-3",

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AU - Sutherland, B. M.

AU - Sutherland, J. C.

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Y1 - 1969

N2 - The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S1) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.

AB - The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S1) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.

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JO - Biophysical Journal

JF - Biophysical Journal

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Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes

Abstract

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