TY - JOUR
T1 - Metabolism of endothelin-1 by neuroblastoma x glioma hybrid (NG108-15) cells
AU - Angelova, Krassimira
AU - Ergul, Adviye
AU - Peng, Kou Cheng
AU - Puett, David
PY - 1997/3/28
Y1 - 1997/3/28
N2 - The endothelin (ET) peptides, ET-1, ET-2, and ET-3, as well as the ET(A) and ET(B) receptor subtypes, are known to occur in brain, but there is a dearth of information on the metabolism of these peptides by the central nervous system (CNS). In this study we have investigated the kinetics of ET-1 binding to and dissociation from the hybrid neuroblastoma x glioma cell line, NG108-15, which is known to contain functional ET receptors, and metabolism of bound ET-1. [125I]ET-1 was incubated with cells for various periods of time up to 6 h, and the nature of the radioactivity in the cell medium and lysate was analyzed by reverse phase high performance liquid chromatography (HPLC). It was found that NG108-15 cells are capable of degrading [125I]ET-1 to [125I]Tyr and several fragments of intermediate hydrophobicity; however, a portion of the cell-associated [125I]ET-1 was protected from degradation for several hours.
AB - The endothelin (ET) peptides, ET-1, ET-2, and ET-3, as well as the ET(A) and ET(B) receptor subtypes, are known to occur in brain, but there is a dearth of information on the metabolism of these peptides by the central nervous system (CNS). In this study we have investigated the kinetics of ET-1 binding to and dissociation from the hybrid neuroblastoma x glioma cell line, NG108-15, which is known to contain functional ET receptors, and metabolism of bound ET-1. [125I]ET-1 was incubated with cells for various periods of time up to 6 h, and the nature of the radioactivity in the cell medium and lysate was analyzed by reverse phase high performance liquid chromatography (HPLC). It was found that NG108-15 cells are capable of degrading [125I]ET-1 to [125I]Tyr and several fragments of intermediate hydrophobicity; however, a portion of the cell-associated [125I]ET-1 was protected from degradation for several hours.
KW - Endothelin degradation
KW - Endothelin-1
KW - NG108-15 cells
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UR - http://www.scopus.com/inward/citedby.url?scp=0031003138&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(97)00166-3
DO - 10.1016/S0304-3940(97)00166-3
M3 - Article
C2 - 9143003
AN - SCOPUS:0031003138
SN - 0304-3940
VL - 225
SP - 1
EP - 4
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -