TY - JOUR
T1 - MicroRNA-1 regulates the differentiation of adipose-derived stem cells into cardiomyocyte-like cells
AU - Chen, Can
AU - Yan, Quanxiang
AU - Yan, Yiguang
AU - Ma, Mudi
AU - He, Yuan
AU - Shui, Xiaorong
AU - Yang, Zhigang
AU - Lan, Xiaozhong
AU - Tang, Yao Liang
AU - Lei, Wei
N1 - Funding Information:
This paper was supported by the National Natural Science Foundation of China (nos. 81300035 and 81741129), Collaborative Innovation and Platform Environment Construction Projects of Guangdong Province (2015A050502049), Natural Science Foundation of Guangdong Province (2015A030313520 and 2016A020214017), Research Project of Traditional Chinese Medicine Bureau of Guangdong Province (20151259), and Stem Cell Preclinical Research Projects of the Affiliated Hospital of Guangdong Medical University (2018PSSC004).
Publisher Copyright:
Copyright © 2018 Can Chen et al.
PY - 2018
Y1 - 2018
N2 - Stem cell transplantation is one of most valuable methods in the treatment of myocardial infarction, and adipose-derived stem cells (ASCs) are becoming a hot topic in medical research. Previous studies have shown that ASCs can be differentiated into cardiomyocyte-like cells, but the efficiency and survival rates are low. We investigated the role and mechanism of microRNA-1 (miR-1) in the differentiation of ASCs into cardiomyocyte-like cells. ASCs and cardiomyocytes were isolated from neonatal rats. We constructed lentivirus for overexpressing miR-1 and used DAPT, an antagonist of the Notch1 pathway, for in vitro analyses. We performed cocultures with ASCs and cardiomyocytes. The differentiation efficiency of ASCs was detected by cell-specific surface antigens. Our results showed that miR-1 can promote the expression of Notch1 and reduce the expression of Hes1, a Notch pathway factor, and overexpression of miR-1 can promote the differentiation of ASCs into cardiomyocyte-like cells, which may occur by regulating Notch1 and Hes1.
AB - Stem cell transplantation is one of most valuable methods in the treatment of myocardial infarction, and adipose-derived stem cells (ASCs) are becoming a hot topic in medical research. Previous studies have shown that ASCs can be differentiated into cardiomyocyte-like cells, but the efficiency and survival rates are low. We investigated the role and mechanism of microRNA-1 (miR-1) in the differentiation of ASCs into cardiomyocyte-like cells. ASCs and cardiomyocytes were isolated from neonatal rats. We constructed lentivirus for overexpressing miR-1 and used DAPT, an antagonist of the Notch1 pathway, for in vitro analyses. We performed cocultures with ASCs and cardiomyocytes. The differentiation efficiency of ASCs was detected by cell-specific surface antigens. Our results showed that miR-1 can promote the expression of Notch1 and reduce the expression of Hes1, a Notch pathway factor, and overexpression of miR-1 can promote the differentiation of ASCs into cardiomyocyte-like cells, which may occur by regulating Notch1 and Hes1.
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U2 - 10.1155/2018/7494530
DO - 10.1155/2018/7494530
M3 - Article
AN - SCOPUS:85056133038
SN - 1687-966X
VL - 2018
JO - Stem Cells International
JF - Stem Cells International
M1 - 7494530
ER -