Microtubules growth rate alteration in human endothelial cells

Irina B. Alieva, Evgeny Alexandrovich Zemskov, Igor I. Kireev, Boris A Gorshkov, Dean A. Wiseman, Stephen Matthew Black, Alexander D. Verin

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


To understand how microtubules contribute to the dynamic reorganization of the endothelial cell (EC) cytoskeleton, we established an EC model expressing EB3-GFP, a protein that marks microtubule plus-ends. Using this model, we were able to measure microtubule growth rate at the centrosome region and near the cell periphery of a single human EC and in the EC monolayer. We demonstrate that the majority of microtubules in EC are dynamic, the growth rate of their plus-ends is highest in the internal cytoplasm, in the region of the centrosome. Growth rate of microtubule plus-ends decreases from the cell center toward the periphery. Our data suggest the existing mechanism(s) of local regulation of microtubule plus-ends growth in EC. Microtubule growth rate in the internal cytoplasm of EC in the monolayer is lower than that of single EC suggesting the regulatory effect of cell-cell contacts. Centrosomal microtubule growth rate distribution in single EC indicated the presence of two subpopulations of microtubules with "normal" (similar to those inmonolayer EC) and "fast" (three times asmuch) growth rates. Our results indicate functional interactions between cell-cell contacts and microtubules.

Original languageEnglish (US)
Article number671536
JournalJournal of Biomedicine and Biotechnology
StatePublished - 2010

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis


Dive into the research topics of 'Microtubules growth rate alteration in human endothelial cells'. Together they form a unique fingerprint.

Cite this