TY - JOUR
T1 - Mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells following their exposure to a discoloration-free calcium aluminosilicate cement
AU - Niu, Li na
AU - Pei, Dan dan
AU - Morris, Matthew
AU - Jiao, Kai
AU - Huang, Xue qing
AU - Primus, Carolyn M.
AU - Ferreira Susin, Lisiane
AU - Bergeron, Brian Edward
AU - Pashley, David Henry
AU - Tay, Franklin Chi Meng
N1 - Funding Information:
Supported by grant R44 DE20204-02 from the National Institute of Dental and Craniofacial Research , grant 2015AA020942 from National High Technology Research and Development Program of China , grant 81400555, 81300898 and 81400551 from NSFC and grant 2015JM8383 from Natural Science Basic Research Plan in Shaanxi Province of China. The authors thank Mrs. Michelle Barnes for her secretarial support.
Publisher Copyright:
© 2016
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Objectives An experimental discoloration-free calcium aluminosilicate cement has been developed with the intention of maximizing the beneficial attributes of tricalcium silicate cements and calcium aluminate cements. The present study examined the effects of this experimental cement (Quick-Set2) on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available tricalcium silicate cement (white mineral trioxide aggregate (ProRoot® MTA); WMTA). Methods The osteogenic potential of hDPSCs exposed to the cements was examined using qRT-PCR for osteogenic gene expressions, Western blot for osteogenic-related protein expressions, alkaline phosphatase enzyme activity, Alizarin red S staining, Fourier transform infrared spectroscopy and transmission electron microscopy of extracellular calcium deposits. Results Results of the six assays indicated that osteogenic differentiation of hDPSCs was significantly enhanced after exposure to the tricalcium silicate cement or the experimental calcium aluminosilicate cement, with the former demonstrating better mineralogenic stimulation capacity. Significance The better osteogenic stimulating effect of the tricalcium silicate cement on hDPSCs may be due to its relatively higher silicate content, or higher OH− and Ca2+ release. Further investigations with the use of in vivo animal models are required to validate the potential augmenting osteogenic effects of the experimental discoloration-free calcium aluminosilicate cement.
AB - Objectives An experimental discoloration-free calcium aluminosilicate cement has been developed with the intention of maximizing the beneficial attributes of tricalcium silicate cements and calcium aluminate cements. The present study examined the effects of this experimental cement (Quick-Set2) on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available tricalcium silicate cement (white mineral trioxide aggregate (ProRoot® MTA); WMTA). Methods The osteogenic potential of hDPSCs exposed to the cements was examined using qRT-PCR for osteogenic gene expressions, Western blot for osteogenic-related protein expressions, alkaline phosphatase enzyme activity, Alizarin red S staining, Fourier transform infrared spectroscopy and transmission electron microscopy of extracellular calcium deposits. Results Results of the six assays indicated that osteogenic differentiation of hDPSCs was significantly enhanced after exposure to the tricalcium silicate cement or the experimental calcium aluminosilicate cement, with the former demonstrating better mineralogenic stimulation capacity. Significance The better osteogenic stimulating effect of the tricalcium silicate cement on hDPSCs may be due to its relatively higher silicate content, or higher OH− and Ca2+ release. Further investigations with the use of in vivo animal models are required to validate the potential augmenting osteogenic effects of the experimental discoloration-free calcium aluminosilicate cement.
KW - Calcium aluminosilicate cement
KW - Human dental pulp stem cells
KW - Mineral trioxide aggregate
KW - Osteogenic differentiation
UR - http://www.scopus.com/inward/record.url?scp=84991065606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991065606&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2016.07.007
DO - 10.1016/j.dental.2016.07.007
M3 - Article
C2 - 27497745
AN - SCOPUS:84991065606
SN - 0109-5641
VL - 32
SP - 1235
EP - 1247
JO - Dental Materials
JF - Dental Materials
IS - 10
ER -