Mitochondrial biogenesis induced by the β2-adrenergic receptor agonist formoterol accelerates podocyte recovery from glomerular injury

Ehtesham Arif, Ashish K. Solanki, Pankaj Srivastava, Bushra Rahman, Wayne R. Fitzgibbon, Peifeng Deng, Milos N. Budisavljevic, Catalin F. Baicu, Michael R. Zile, J. Megyesi, Michael G. Janech, Sang Ho Kwon, Justin Collier, Rick G. Schnellmann, Deepak Nihalani

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Podocytes have limited ability to recover from injury. Here, we demonstrate that increased mitochondrial biogenesis, to meet the metabolic and energy demand of a cell, accelerates podocyte recovery from injury. Analysis of events induced during podocyte injury and recovery showed marked upregulation of peroxisome proliferator-activated receptor-γ coactivator–1α (PGC-1α), a transcriptional co-activator of mitochondrial biogenesis, and key components of the mitochondrial electron transport chain. To evaluate our hypothesis that increasing mitochondrial biogenesis enhanced podocyte recovery from injury, we treated injured podocytes with formoterol, a potent, specific, and long-acting β2-adrenergic receptor agonist that induces mitochondrial biogenesis in vitro and in vivo. Formoterol increased mitochondrial biogenesis and restored mitochondrial morphology and the injury-induced changes to the organization of the actin cytoskeleton in podocytes. Importantly, β2-adrenergic receptors were found to be present on podocyte membranes. Their knockdown attenuated formoterol-induced mitochondrial biogenesis. To determine the potential clinical relevance of these findings, mouse models of acute nephrotoxic serum nephritis and chronic (Adriamycin [doxorubicin]) glomerulopathy were used. Mice were treated with formoterol post-injury when glomerular dysfunction was established. Strikingly, formoterol accelerated the recovery of glomerular function by reducing proteinuria and ameliorating kidney pathology. Furthermore, formoterol treatment reduced cellular apoptosis and increased the expression of the mitochondrial biogenesis marker PGC-1α and multiple electron transport chain proteins. Thus, our results support β2-adrenergic receptors as novel therapeutic targets and formoterol as a therapeutic compound for treating podocytopathies.

Original languageEnglish (US)
Pages (from-to)656-673
Number of pages18
JournalKidney International
Issue number3
StatePublished - Sep 2019


  • albuminuria
  • focal segmental glomerulosclerosis
  • glomerulonephritis
  • glomerulus
  • podocyte

ASJC Scopus subject areas

  • Nephrology


Dive into the research topics of 'Mitochondrial biogenesis induced by the β2-adrenergic receptor agonist formoterol accelerates podocyte recovery from glomerular injury'. Together they form a unique fingerprint.

Cite this