TY - JOUR
T1 - Mitochondrial DNA Haplotype Diversity in Two Portunid Populations Found along the Georgia and South Carolina Coasts
AU - Abdulovic-Cui, Amy
AU - Mannix, Sierra
AU - Coleman, Austin
AU - White, Brittany
AU - Mingledolph, Keturah
AU - Reichmuth, Jessica M.
N1 - Funding Information:
DNA extraction and sequencing was supported by a Georgia Department of Natural Resources/Coastal Resources Division Coastal Incentive Grant (Grant # NA15NOS4190160). Funding was also provided by many sources at Augusta University, and without this financial support, parts of this project would not have been possible: Department of Biological Sciences, the Center for Undergraduate Research and Scholarship, and the Pamplin/College of Science and Mathematics Student Travel Fund. Two undergraduates, specifically April Hammack and Montel Hughes, spent many hours dissecting crab limbs and preparing tissue for DNA extractions as well as aided in data analysis. Many thanks to the Gritty Britches Club, a large group of undergraduate student volunteers that spent extended hours seining for crabs, pulling crabs out of crab pots, and occasionally getting pinched—without their help, specimen collection would have been difficult. Travel to collection sites within the SGA site would not have been possible without the many hours contributed by local landowners in their personal boats: special thanks to Fred Voigt Jr., Fred Voigt III, Tommy Zachry, Wilton DeLoach, Clay Montague, and Admiral Lee Watkins. The comments of the
Publisher Copyright:
© 2021 Humboldt Field Research Institute. All rights reserved.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Habitat loss, overfishing, and decline in abundance and quality of natural resources continue to threaten marine ecosystems. A common tool used to measure health and diversity within a system is genetic diversity. The purpose of this study was to determine the genetic diversity of 2 crustacean populations by sequencing the 16S rRNA mitochondrial loci. We collected Callinectes sapidus (Blue Crab) and Arenaeus cribrarius (Speckled Swimming Crab) using seines and standard crab pots from 2 study sites along the Georgia and South Carolina coasts. We extracted DNA from leg or claw tissue and used previously published primers to sequence the 16S rRNA loci of the mitochondria and record the number of mutations found from individual crabs. A total of 177 crabs (Blue Crab: n = 129; Speckled Swimming Crab: n = 48) were sequenced. Overall, the Blue Crab showed more genetic diversity relative to the Speckled Swimming Crab, but this result may due to sample size. Knowledge of the genetic diversity of an economically important species has implications for management.
AB - Habitat loss, overfishing, and decline in abundance and quality of natural resources continue to threaten marine ecosystems. A common tool used to measure health and diversity within a system is genetic diversity. The purpose of this study was to determine the genetic diversity of 2 crustacean populations by sequencing the 16S rRNA mitochondrial loci. We collected Callinectes sapidus (Blue Crab) and Arenaeus cribrarius (Speckled Swimming Crab) using seines and standard crab pots from 2 study sites along the Georgia and South Carolina coasts. We extracted DNA from leg or claw tissue and used previously published primers to sequence the 16S rRNA loci of the mitochondria and record the number of mutations found from individual crabs. A total of 177 crabs (Blue Crab: n = 129; Speckled Swimming Crab: n = 48) were sequenced. Overall, the Blue Crab showed more genetic diversity relative to the Speckled Swimming Crab, but this result may due to sample size. Knowledge of the genetic diversity of an economically important species has implications for management.
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U2 - 10.1656/058.020.0214
DO - 10.1656/058.020.0214
M3 - Article
AN - SCOPUS:85115161412
SN - 1528-7092
VL - 20
SP - 367
EP - 376
JO - Southeastern Naturalist
JF - Southeastern Naturalist
IS - 2
ER -