Molecular cloning of cDNA for rat brain GD3-synthase

Guichao Zeng, Luoyi Gao, Toshio Ariga, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Sep 13 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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