Molecular cloning of cDNA for rat brain GD3-synthase

Guichao Zeng; Luoyi Gao; Toshio Ariga; Robert K. Yu

doi:10.1006/bbrc.1996.1354

Molecular cloning of cDNA for rat brain GD3-synthase

Guichao Zeng, Luoyi Gao, Toshio Ariga, Robert K. Yu

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.

Original language	English (US)
Pages (from-to)	319-323
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	226
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.1354
State	Published - Sep 13 1996
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1996.1354

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title = "Molecular cloning of cDNA for rat brain GD3-synthase",

abstract = "The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.",

author = "Guichao Zeng and Luoyi Gao and Toshio Ariga and Yu, {Robert K.}",

note = "Funding Information: This work was supported by USPHS Grant NS11853.",

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PY - 1996/9/13

Y1 - 1996/9/13

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Molecular cloning of cDNA for rat brain GD3-synthase

Abstract

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