Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome

Mark D. Johnson; Sandra P.T. Tho; Ali Behzadian; Paul G. McDonough

doi:10.1016/0002-9378(89)90959-9

Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome

Mark D. Johnson, Sandra P.T. Tho, Ali Behzadian, Paul G. McDonough

Physiology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical, for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes Taql, EcoRl, and BamHl. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.

Original language	English (US)
Pages (from-to)	1732-1737
Number of pages	6
Journal	American journal of obstetrics and gynecology
Volume	161
Issue number	6 PART 1
DOIs	https://doi.org/10.1016/0002-9378(89)90959-9
State	Published - Dec 1989

Keywords

DNA
Sertoli-cell-only syndrome
Spermatogenesis
Y chromosome

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/0002-9378(89)90959-9

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title = "Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome",

abstract = "Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical, for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes Taql, EcoRl, and BamHl. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.",

keywords = "DNA, Sertoli-cell-only syndrome, Spermatogenesis, Y chromosome",

author = "Johnson, {Mark D.} and Tho, {Sandra P.T.} and Ali Behzadian and McDonough, {Paul G.}",

year = "1989",

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doi = "10.1016/0002-9378(89)90959-9",

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volume = "161",

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T1 - Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome

AU - Johnson, Mark D.

AU - Tho, Sandra P.T.

AU - Behzadian, Ali

AU - McDonough, Paul G.

PY - 1989/12

Y1 - 1989/12

N2 - Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical, for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes Taql, EcoRl, and BamHl. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.

AB - Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical, for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes Taql, EcoRl, and BamHl. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.

KW - DNA

KW - Sertoli-cell-only syndrome

KW - Spermatogenesis

KW - Y chromosome

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JO - American journal of obstetrics and gynecology

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IS - 6 PART 1

ER -

Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome

Abstract

Keywords

ASJC Scopus subject areas

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