TY - JOUR
T1 - Monitoring forced degradation of drugs using silica coated AgNPs with surface-enhanced Raman scattering
AU - Yilmaz, Hulya
AU - Cobandede, Zehra
AU - Yilmaz, Deniz
AU - Cinkilic, Ayse
AU - Culha, Mustafa
AU - Demiralay, Ebru Cubuk
N1 - Funding Information:
The financial support from Yeditepe University is greatly apppeciated.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Potential degradation products (DPs), even in small concentrations, can cause changes in pharmacological and toxicological properties of a drug with a significant impact on product quality and safety. Thus, their stability and understanding of possible degradation mechanisms have a significant importance. Although liquid chromatography is the conventional technique used for forced degradation studies with excellent accuracy and reproducibility, the main disadvantages of the technique are being expensive and time-consuming. As a powerful technique, surface-enhanced Raman scattering (SERS) can be an alternative with its high sensitivity, easy sample preparation and low cost. In this study, the degradation of both tofacitinib (TOF), a Janus kinase inhibitor, and methotrexate (MTX), an inhibitor of tetrahydrofolate dehydrogenase, are studied using SERS under hydrolytic, oxidative and thermal conditions using mesoporous silica coated silver nanoparticles (Si@AgNPs) as SERS substrates. The study demonstrated that the degradation of the tested drugs using Si@AgNPs as SERS substrates could be monitored through the spectral changes on SERS spectra of drugs under several degradation conditions.
AB - Potential degradation products (DPs), even in small concentrations, can cause changes in pharmacological and toxicological properties of a drug with a significant impact on product quality and safety. Thus, their stability and understanding of possible degradation mechanisms have a significant importance. Although liquid chromatography is the conventional technique used for forced degradation studies with excellent accuracy and reproducibility, the main disadvantages of the technique are being expensive and time-consuming. As a powerful technique, surface-enhanced Raman scattering (SERS) can be an alternative with its high sensitivity, easy sample preparation and low cost. In this study, the degradation of both tofacitinib (TOF), a Janus kinase inhibitor, and methotrexate (MTX), an inhibitor of tetrahydrofolate dehydrogenase, are studied using SERS under hydrolytic, oxidative and thermal conditions using mesoporous silica coated silver nanoparticles (Si@AgNPs) as SERS substrates. The study demonstrated that the degradation of the tested drugs using Si@AgNPs as SERS substrates could be monitored through the spectral changes on SERS spectra of drugs under several degradation conditions.
KW - Drug
KW - Forced degradation
KW - Silica coated silver nanoparticles
KW - Surface-enhanced Raman scattering
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U2 - 10.1016/j.talanta.2020.120828
DO - 10.1016/j.talanta.2020.120828
M3 - Article
C2 - 32278418
AN - SCOPUS:85079884418
SN - 0039-9140
VL - 214
JO - Talanta
JF - Talanta
M1 - 120828
ER -