TY - JOUR
T1 - Monocyte activation in patients with age-related macular degeneration
T2 - A biomarker of risk for choroidal neovascularization?
AU - Cousins, Scott W.
AU - Espinosa-Heidmann, Diego G.
AU - Csaky, Karl G.
PY - 2004/7
Y1 - 2004/7
N2 - Objective: To evaluate the activation state of macrophage function in patients with age-related macular degeneration (AMD) by quantifying the production of the proinflammatory and angiogenic factor tumor necrosis factor α (TNF-α) and by correlating its expression with dry and wet AMD. Methods: Circulating monocytes were obtained from the blood of patients with AMD or age-matched control subjects by gradient centrifugation. The monocytes were then analyzed for either TNF-α release from cultured macrophages in response to retinal pigment epithelium-derived blebs and cytokines or TNF-α messenger RNA content by reverse transcriptase-polymerase chain reaction. Results: In human monocytes obtained from controls and AMD patients, TNF-α was expressed by freshly isolated monocytes and produced by macrophages in culture after stimulation with retinal pigment epithelium-derived blebs. However, wide variability in TNF-α expression was observed among different patients. Patients with monocytes that expressed the greatest amount of TNF-α demonstrated higher prevalence of choroidal neovascularization. Conclusions: Both controls and AMD patients vary in the activation state (defined as TNF-α expression) of circulating monocytes. Partially active monocytes, defined as high TNF-α expression, may be a biomarker to identify patients at risk for formation of choroidal neovascularization. Clinical of Relevance: Early diagnostic testing may prove useful to detect those patients who will progress to the more severe complications of the disease.
AB - Objective: To evaluate the activation state of macrophage function in patients with age-related macular degeneration (AMD) by quantifying the production of the proinflammatory and angiogenic factor tumor necrosis factor α (TNF-α) and by correlating its expression with dry and wet AMD. Methods: Circulating monocytes were obtained from the blood of patients with AMD or age-matched control subjects by gradient centrifugation. The monocytes were then analyzed for either TNF-α release from cultured macrophages in response to retinal pigment epithelium-derived blebs and cytokines or TNF-α messenger RNA content by reverse transcriptase-polymerase chain reaction. Results: In human monocytes obtained from controls and AMD patients, TNF-α was expressed by freshly isolated monocytes and produced by macrophages in culture after stimulation with retinal pigment epithelium-derived blebs. However, wide variability in TNF-α expression was observed among different patients. Patients with monocytes that expressed the greatest amount of TNF-α demonstrated higher prevalence of choroidal neovascularization. Conclusions: Both controls and AMD patients vary in the activation state (defined as TNF-α expression) of circulating monocytes. Partially active monocytes, defined as high TNF-α expression, may be a biomarker to identify patients at risk for formation of choroidal neovascularization. Clinical of Relevance: Early diagnostic testing may prove useful to detect those patients who will progress to the more severe complications of the disease.
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U2 - 10.1001/archopht.122.7.1013
DO - 10.1001/archopht.122.7.1013
M3 - Article
C2 - 15249366
AN - SCOPUS:3342974773
SN - 0003-9950
VL - 122
SP - 1013
EP - 1018
JO - Archives of Ophthalmology
JF - Archives of Ophthalmology
IS - 7
ER -