Abstract
Crohn’s disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Chronic intestinal inflammation is inextricably linked to colitis-associated colon carcinogenesis (CAC). Patients with ulcerative colitis (UC) and Crohn’s disease (CD) have an increased risk of colon cancer. Our understanding of IBD and IBD-associated colon carcinogenesis depends largely on rodent models. AOM-DSS-induced colitis-associated colon cancer in mice is the most widely used and accepted model that can recapitulate the human IBD-associated colon cancer. Here, we have provided detailed protocols of this mouse model of experimentally induced chronic intestinal inflammation-associated colon cancer. We will also discuss the protocols for the isolation and analysis of inflammatory immune cells from the colon.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 133-146 |
| Number of pages | 14 |
| DOIs | |
| State | Published - 2021 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2224 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- AOM
- CAC
- Chronic colitis
- DSS
- Flow cytometry and intestinal immune cells
- IBD-associated colon cancer
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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