TY - JOUR
T1 - Murine and rat cavernosal responses to endothelin-1 and urotensin-II Vasoactive Peptide Symposium
AU - Carneiro, Fernando S.
AU - Carneiro, Zidonia N.
AU - Giachini, Fernanda R.C.
AU - Lima, Victor V.
AU - Nogueira, Edson F.
AU - Rainey, William E.
AU - Tostes, Rita C.
AU - Webb, R. Clinton
N1 - Funding Information:
This study was supported by Grants from the National Institutes of Health (HL71138 and HL74167), Bethesda, Maryland and Fundacao de Amparo a Pesquisa do Estado de São Paulo, FAPESP, Brazil.
PY - 2008/11
Y1 - 2008/11
N2 - Endothelin-1 (ET-1) and urotensin-II (U-II) are the most potent constrictors of human vessels. Although the cavernosal tissue is highly responsive to ET-1, no information exists on the effects of U-II on cavernosal function. The aim of this study was to characterize ET-1 and U-II responses in corpora cavernosa from rats and mice. Male Wistar rats and C57/BL6 mice were used at 13 weeks. Cumulative concentration-response curves to ET-1, U-II, and IRL-1620, an ETB agonist, were performed. ET-1 increased force generation in cavernosal strips from mice and rats, but no response to U-II was observed in the presence or absence of Nω-nitro-L-arginine methyl ester (L-NAME), or in strips prestimulated with 20 mM KCl. IRL-1620 did not induce cavernosal contraction even in presence of L-NAME, but induced a cavernosal relaxation that was greater in rats than mice. No relaxation responses to U-II were observed in cavernosal strips precontracted with phenylephrine. mRNA expression of ET-1, ETA, ETB, and U-II receptors, but not U-II was observed in cavernosal strips. ET-1, via ETA receptors activation, causes contractile responses in cavernosal strips from rats and mice, whereas ETB receptor activation produces relaxation. Although the cavernosal tissue expresses U-II receptors, U-II does not induce contractile responses in corpora cavernosa from mice or rats.
AB - Endothelin-1 (ET-1) and urotensin-II (U-II) are the most potent constrictors of human vessels. Although the cavernosal tissue is highly responsive to ET-1, no information exists on the effects of U-II on cavernosal function. The aim of this study was to characterize ET-1 and U-II responses in corpora cavernosa from rats and mice. Male Wistar rats and C57/BL6 mice were used at 13 weeks. Cumulative concentration-response curves to ET-1, U-II, and IRL-1620, an ETB agonist, were performed. ET-1 increased force generation in cavernosal strips from mice and rats, but no response to U-II was observed in the presence or absence of Nω-nitro-L-arginine methyl ester (L-NAME), or in strips prestimulated with 20 mM KCl. IRL-1620 did not induce cavernosal contraction even in presence of L-NAME, but induced a cavernosal relaxation that was greater in rats than mice. No relaxation responses to U-II were observed in cavernosal strips precontracted with phenylephrine. mRNA expression of ET-1, ETA, ETB, and U-II receptors, but not U-II was observed in cavernosal strips. ET-1, via ETA receptors activation, causes contractile responses in cavernosal strips from rats and mice, whereas ETB receptor activation produces relaxation. Although the cavernosal tissue expresses U-II receptors, U-II does not induce contractile responses in corpora cavernosa from mice or rats.
KW - DOCA-salt hypertension
KW - ET receptor
KW - corpus cavernosum
KW - erectile dysfunction
UR - http://www.scopus.com/inward/record.url?scp=57149092118&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57149092118&partnerID=8YFLogxK
U2 - 10.1016/j.jash.2008.07.001
DO - 10.1016/j.jash.2008.07.001
M3 - Article
AN - SCOPUS:57149092118
SN - 1933-1711
VL - 2
SP - 439
EP - 447
JO - Journal of the American Society of Hypertension
JF - Journal of the American Society of Hypertension
IS - 6
ER -