TY - JOUR
T1 - Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein
AU - Huang, Pin
AU - Lu, Chunwan
AU - Li, Jun
AU - Xu, Jun
AU - Liu, Zaiqun
AU - Wang, Qiang
AU - Wang, Zhao
AU - Huo, Jiayan
AU - Li, Honglin
AU - Teng, Yong
AU - Cai, Yafei
N1 - Funding Information:
This study was supported by the National Natural Science Foundation of China ( 31372207 ); the National Supporting Projects for Science and Techniques ( 2012BAD12B10 and 2012BAD12B1003 ); and the Anhui Natural Science and Technology Program of Anhui Province ( 1208085MC41 & KJ2013A310 ).
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/3/15
Y1 - 2015/3/15
N2 - To select the molecular markers susceptible to mastitis and reduce the loss induced by mastitis, the PCR-SSCP method was adopted to investigate the correlation between SNPs of the HSP70-2 gene and mastitis in 103 Chinese Holstein. 25 new polymorphisms were detected in this study: 9 SNPs (g- 115 G → A, g- 98 T → C, g.117 C → A, g.156 A → C, g.1743 G → A, g.1746 C → T, g.1761 T → G, g.1770 C → G, g.1877 G → C) were found to be associated with mastitis; 2 key SNPs led to amino-acid changes: g.1770 C → G (590 Asp → Glu), g.1877 G → C (626 Gly → Ala). 626 Gly → Ala affected the protein secondary structure. 3 potential cow HSP70-2 proteins were found in all the 103 individuals, but predicted three-dimensional structures of 3 proteins are the same with each other. It is suggested that 9 SNPs increase the susceptibility to mastitis due to their low polymorphisms and can be used as molecular markers to breed the dairy cows resistant to mastitis.
AB - To select the molecular markers susceptible to mastitis and reduce the loss induced by mastitis, the PCR-SSCP method was adopted to investigate the correlation between SNPs of the HSP70-2 gene and mastitis in 103 Chinese Holstein. 25 new polymorphisms were detected in this study: 9 SNPs (g- 115 G → A, g- 98 T → C, g.117 C → A, g.156 A → C, g.1743 G → A, g.1746 C → T, g.1761 T → G, g.1770 C → G, g.1877 G → C) were found to be associated with mastitis; 2 key SNPs led to amino-acid changes: g.1770 C → G (590 Asp → Glu), g.1877 G → C (626 Gly → Ala). 626 Gly → Ala affected the protein secondary structure. 3 potential cow HSP70-2 proteins were found in all the 103 individuals, but predicted three-dimensional structures of 3 proteins are the same with each other. It is suggested that 9 SNPs increase the susceptibility to mastitis due to their low polymorphisms and can be used as molecular markers to breed the dairy cows resistant to mastitis.
KW - Chinese Holstein
KW - HSP70-2
KW - Mastitis
KW - SNPs
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U2 - 10.1016/j.gene.2015.01.015
DO - 10.1016/j.gene.2015.01.015
M3 - Article
C2 - 25592821
AN - SCOPUS:84922215639
SN - 0378-1119
VL - 559
SP - 62
EP - 72
JO - Gene
JF - Gene
IS - 1
ER -