Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression

Richard S. Cameron, Changdan Liu, April S. Mixon, Jeanene P.S. Pihkala, Rebecca J. Rahn, Patricia L. Cameron

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Rat Myo16a and Myo16b comprise the founding members of class XVI myosin and are characterized by an N-terminal ankyrin repeat domain thought to mediate an association with protein phosphatase 1 catalytic subunits 1α and 1γ. Myo16b is the principal isoform and reveals predominant expression in developing neural tissue. Here, we use COS-7 cells as a model system to develop an understanding of Myo16b function. We find that Myo16b displays predominant localization in the nucleus of cells transitioning through interphase, but is not associated with processes of mitosis. Using a panel of EGFP-Myo16b- expression plasmids in transient transfection studies, we identified the COOH-terminal residues 1616-1912 as necessary and solely sufficient to target Myo16b to the nucleus. We show that the Myo16b-tail region directs localization to a nuclear compartment containing profilin and polymerized actin, which appears to form a three-dimensional meshwork through the depth of the nucleus. Further, we demonstrate that this compartment localizes within euchromatic regions of the genome and contains proliferating cell nuclear antigen (PCNA) and cyclin A. both markers of S-phase of the cell cycle. Cells transiently expressing Myo16b or Myo16b-tail region show limited incorporation of BrdU, delayed progression through S-phase of the cell cycle, and curtailed cellular proliferation.

Original languageEnglish (US)
Pages (from-to)19-48
Number of pages30
JournalCell Motility and the Cytoskeleton
Issue number1
StatePublished - Jan 2007


  • Actin
  • Brain development
  • Subnuclear foci
  • Unconventional myosin

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology


Dive into the research topics of 'Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression'. Together they form a unique fingerprint.

Cite this