Abstract
Embryonic stem cells (ES cells) are developmentally pluripotent cells isolated from pre-implantation mammalian embryos. In cell culture ES cells can be easily differentiated to generate cultures of neural progenitors. We present a simple method for the cryopreservation of these ES-derived neural progenitors. Cryopreserved neural progenitor stocks can be thawed, expanded with FGF2, and differentiated into functional neurons. This method will facilitate studies using ES-derived neural progenitor cells as a cell culture model system for neural development and differentiation. It will also aid studies designed to test the ability of these progenitor cells to functionally engraft and repair damaged neural tissue. (C) 2000 Academic Press.
Original language | English (US) |
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Pages (from-to) | 418-421 |
Number of pages | 4 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 271 |
Issue number | 2 |
DOIs | |
State | Published - May 10 2000 |
Keywords
- Cell culture
- Cryopreservation
- Embryonic stem cells
- Neural stem cells
- Neuronal differentiation
- Pluripotent stem cells
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology