Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

Goldin John; Nikhil Shri Sahajpal; Ashis K Mondal; Sudha Ananth; Colin Williams; Alka Chaubey; Amyn M Rojiani; Ravindra Kolhe

doi:10.3390/cimb43020061

Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

Goldin John, Nikhil Shri Sahajpal, Ashis K Mondal, Sudha Ananth, Colin Williams, Alka Chaubey, Amyn M Rojiani, Ravindra Kolhe

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.

Original language	English (US)
Pages (from-to)	845-867
Number of pages	23
Journal	Current Issues in Molecular Biology
Volume	43
Issue number	2
DOIs	https://doi.org/10.3390/cimb43020061
State	Published - Jul 30 2021

Keywords

COVID-19/diagnosis
Computational Biology/methods
High-Throughput Nucleotide Sequencing/methods
Humans
Molecular Epidemiology/methods
Pandemics
Phylogeny
SARS-CoV-2/genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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John, G., Sahajpal, N. S., Mondal, A. K., Ananth, S., Williams, C., Chaubey, A., Rojiani, A. M., & Kolhe, R. (2021). Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology. Current Issues in Molecular Biology, 43(2), 845-867. https://doi.org/10.3390/cimb43020061

Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology. / John, Goldin; Sahajpal, Nikhil Shri; Mondal, Ashis K et al.
In: Current Issues in Molecular Biology, Vol. 43, No. 2, 30.07.2021, p. 845-867.

Research output: Contribution to journal › Article › peer-review

John, G, Sahajpal, NS, Mondal, AK, Ananth, S, Williams, C, Chaubey, A, Rojiani, AM & Kolhe, R 2021, 'Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology', Current Issues in Molecular Biology, vol. 43, no. 2, pp. 845-867. https://doi.org/10.3390/cimb43020061

John G, Sahajpal NS, Mondal AK, Ananth S, Williams C, Chaubey A et al. Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology. Current Issues in Molecular Biology. 2021 Jul 30;43(2):845-867. doi: 10.3390/cimb43020061

John, Goldin ; Sahajpal, Nikhil Shri ; Mondal, Ashis K et al. / Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology : A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology. In: Current Issues in Molecular Biology. 2021 ; Vol. 43, No. 2. pp. 845-867.

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Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

Abstract

Keywords

UN SDGs

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